| Products of cultured neuroglial cells: II. The production of fibronectin by C6 glioma cells. | |
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MedLine Citation:
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PMID: 3892349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The possibility of fibronectin production by C6 glioma cells was examined with assays which require protein synthesis. Proteins produced by C6 cells using radiolabeled amino acid precursors were tested for affinity to collagen by binding to immobilized gelatin. The predominant collagen binding protein made by C6 coelectrophoresed with fibronectin synthesized by control fibroblasts and with the larger of the two proteins in unlabeled fibronectin when applied to polyacrylamide gels with sodium dodecyl sulfate (SDS). In addition, C6 produced a larger collagen binding protein of approximately 270,000 molecular weight. Solubilities in urea solutions of the collagen-binding proteins made by C6 cells and fibroblasts were similar. Immunofluorescence showed fibronectin associated with the C6 cell monolayer, but less abundant than the fibronectin associated with fibroblasts. Results provide evidence for the production of fibronectin by the C6 glioma cell line. |
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Authors:
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J R Baldwin; P E McKeever; T R Booker |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neurochemical research Volume: 10 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 1985 May |
Date Detail:
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Created Date: 1985-08-22 Completed Date: 1985-08-22 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 601-10 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Collagen / metabolism Electrophoresis, Polyacrylamide Gel Fibroblasts / metabolism Fibronectins / biosynthesis* Fluorescent Antibody Technique Glioma / metabolism* Leucine / metabolism Molecular Weight Rats Solubility Urea |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 57-13-6/Urea; 61-90-5/Leucine; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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