Document Detail


Productive infection of normal CD40-activated human B lymphocytes by HIV-1.
MedLine Citation:
PMID:  7531456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Antigen-driven B-cell proliferation and maturation occur in germinal centres present in lymphoid tissues. This process is highly dependent on functional interactions between B and T lymphocytes. In vitro activation of CD40 present on B cells mimics B cell-T interactions and allows the proliferation of normal Epstein-Barr virus (EBV)-negative B lymphocytes. In HIV-1-seropositive individuals, B cells become exposed to free viral particles and to infected T lymphocytes while migrating through germinal centres. The effect of HIV-1 viral exposure on CD40-activated B lymphocytes was therefore examined. METHODS: Freshly isolated B lymphocytes were cultured in vitro through activation of CD40. B-cell proliferation, HIV-1 infectivity and viral production were monitored following B-lymphocyte exposure to HIV-1. In addition, HIV-mediated fusion between infected B cells and uninfected CD4+ T lymphocytes was assessed in a coculture assay. RESULTS: EBV-negative, CD40-activated human B lymphocytes were directly infected by HIV-1. The infection significantly reduced their proliferation rate. Viral production was detected in B-cell culture supernatant. Numerous fusion events indicated that HIV-1 infection of B lymphocytes could spread to T lymphocytes following HIV-1-mediated fusion of these two cell types. CONCLUSION: In view of the importance of B cell-T cell interactions in the maintenance of a functional immune system, disruption of B-lymphocyte development could have direct implications on the course of AIDS progression.
Authors:
L Poulin; N Paquette; S Moir; R Lapointe; A Darveau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  AIDS (London, England)     Volume:  8     ISSN:  0269-9370     ISO Abbreviation:  AIDS     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-03-14     Completed Date:  1995-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8710219     Medline TA:  AIDS     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1539-44     Citation Subset:  IM; X    
Affiliation:
Department of Microbiology, Laval University, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / immunology*
Antigens, CD40
Antigens, Differentiation, B-Lymphocyte / immunology*
B-Lymphocytes / immunology*,  virology*
CD4-Positive T-Lymphocytes / immunology
Cells, Cultured
DNA / biosynthesis
DNA, Viral / analysis,  biosynthesis
Fluorescent Antibody Technique
Genes, env
Genes, gag
Genes, pol
HIV Seronegativity / immunology
HIV-1 / genetics,  immunology*,  physiology
Humans
Lymphocyte Activation
Polymerase Chain Reaction
T-Lymphocytes / immunology
Virus Replication
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD40; 0/Antigens, Differentiation, B-Lymphocyte; 0/DNA, Viral; 9007-49-2/DNA

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