| Production of vasoactive substances by human umbilical vein endothelial cells after incubation with serum from preeclamptic patients. | |
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MedLine Citation:
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PMID: 11788166 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the in vitro effect of serum from preeclamptic patients on the proliferation, viability and secretion of vasoactive substances by human umbilical vein endothelial cells (HUVEC). STUDY DESIGN: HUVEC were incubated for 24h with sera from 16 preeclamptic, 19 healthy pregnant and 8 healthy nonpregnant women. Proliferation rates were determined by cell counting and vitality by trypan blue staining. The vasoactive substances, 6-keto-prostaglandin F(1alpha), nitrite and nitrate, and endothelin-1 (ET-1) were measured in endothelial cell supernatants. RESULTS: The preeclamptic serum had no effect on cell proliferation or vitality compared with control sera. It induced more HUVEC production of ET-1, but not of prostacyclin (PGI2) or nitric oxide compared with control serum. CONCLUSION: Preeclamptic serum appears to contain a factor(s) that specifically stimulates ET-1 secretion from HUVEC without altering cell growth or vitality. |
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Authors:
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F Scalera; D Schlembach; E Beinder |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of obstetrics, gynecology, and reproductive biology Volume: 99 ISSN: 0301-2115 ISO Abbreviation: Eur. J. Obstet. Gynecol. Reprod. Biol. Publication Date: 2001 Dec |
Date Detail:
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Created Date: 2002-01-14 Completed Date: 2002-03-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375672 Medline TA: Eur J Obstet Gynecol Reprod Biol Country: Ireland |
Other Details:
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Languages: eng Pagination: 172-8 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Erlangen/Nuremberg, Universitaetsstr. 2-23, 91054, Erlangen, Germany. fortunato.scalera@gyn.med.uni-erlangen.de |
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| MeSH Terms | |
Descriptor/Qualifier:
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6-Ketoprostaglandin F1 alpha
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biosynthesis*,
secretion Adult Blood Cell Division Cells, Cultured Culture Media Endothelin-1 / biosynthesis*, secretion Endothelium, Vascular / metabolism* Female Humans Nitrates / metabolism Nitric Oxide / biosynthesis*, secretion Nitrites / metabolism Pre-Eclampsia / blood* Pregnancy Umbilical Veins |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Endothelin-1; 0/Nitrates; 0/Nitrites; 10102-43-9/Nitric Oxide; 58962-34-8/6-Ketoprostaglandin F1 alpha |
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