Document Detail


Production and utilization of polyclonal antibodies against nisin in an ELISA and for immuno-location of nisin in producing and sensitive bacterial strains.
MedLine Citation:
PMID:  10583677     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Specific nisin polyclonal antibodies (PAb) were produced in rabbits using nisin Z produced by Lactococcus lactis subsp. lactis biovar diacetylactis UL 719. Antisera were obtained from white female New Zealand rabbits that were first immunized with a nisin Z-keyhole limpet haemocyanin conjugate and boosted with free nisin Z. Nisin-specific PAb were purified by affinity chromatography with a yield of 15 mg specific antinisin 100 ml-1 serum. The detection limit of the ELISA test for nisin Z was 0.75 ng ml-1 in buffer but was 1.7 and 3.5 ng ml-1 in milk and complex media broth spiked (5, 10, 20 microg ml-1) with nisin Z, respectively. In nisin Z-spiked samples, the average concentration was between 90 and 107% of actual added amount. In contrast, when the bioassay (microtitration method) was used, only 50-63% of nisin Z biological activity could be detected. In addition, the affinity-purified nisin PAb, antirabbit IgG gold conjugate and transmission electron microscopy were successfully used to locate nisin Z on producing cells and to observe its bactericidal effects against sensitive cells.
Authors:
M Bouksaim; C Lacroix; R Bazin; R E Simard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  87     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  2000-01-18     Completed Date:  2000-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  500-10     Citation Subset:  IM    
Affiliation:
Centre de recherche en sciences et technologie du lait, Université Laval, Sainte-Foy, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriocins / analysis
Chromatography, Affinity / methods
Enzyme-Linked Immunosorbent Assay / methods
Female
Immune Sera / biosynthesis*,  isolation & purification
Immunoglobulin G / biosynthesis
Lactococcus lactis / chemistry*
Microscopy, Electron
Milk / microbiology
Nisin / analogs & derivatives*,  analysis,  immunology
Rabbits
Chemical
Reg. No./Substance:
0/Bacteriocins; 0/Immune Sera; 0/Immunoglobulin G; 137061-46-2/nisin Z; 1414-45-5/Nisin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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