Document Detail

Production of a uniform cellular injury by raster scanning of cells for the study of laser bioeffects.
MedLine Citation:
PMID:  17122415     Owner:  NLM     Status:  MEDLINE    
Efforts to understand laser bioeffects in cells and tissues have been hindered by a nonuniform cellular response of the specimen, resulting in graded biochemical effects. In addition, the small beam diameters of commonly used lasers limit the number of cells expressing a response to numbers inadequate for the study of biochemical effects. For a limited emission power, expansion of the beam diameter reduces the irradiance, thus requiring longer exposure durations to produce a cellular response. Cultured human retinal epithelial cells were exposed as a single spot ("tophat" exposure) from a carbon dioxide (CO(2)) laser operating at 10.6 microm or scanned with a raster system and compared with thermal injury produced with heated saline for short periods (1-9 s) at relatively high temperature (55-70 degrees C). Cell viability and induction of the 70 kDa heat shock protein were evaluated as indicators of the cellular response. Initial attempts to use a tophat (uniform energy distribution) exposure resulted in a nonuniform cellular response (and nonuniform energy distribution) due to diffraction effects from the 2-mm selection aperture. However, raster scanning for appropriate times with the CO(2) laser yielded uniform cell viability and heat shock protein synthesis that were comparable to dipping cells in heated saline. Because scanning results in a homogeneous exposure of cells, the described scanning technique may be applied to studies of cellular responses to other lasers to evaluate photochemical and photomechanical effects.
K P Walker; S T Schuschereba; P R Edsall; B E Stuck; P D Bowman
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Publication Detail:
Type:  Journal Article     Date:  2006-11-22
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  292     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-12     Completed Date:  2007-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C1536-42     Citation Subset:  IM    
U.S. Army Medical Research Detachment, Brooks City--Base, Texas, USA.
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MeSH Terms
Carbon Dioxide
Cell Line
Cell Survival / radiation effects
HSP70 Heat-Shock Proteins / metabolism
Lasers / adverse effects*
Pigment Epithelium of Eye / pathology,  radiation effects*
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 124-38-9/Carbon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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