| Production of trypsins by human gastric cancer cells correlates with their malignant phenotype. | |
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MedLine Citation:
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PMID: 9849464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Proteolytic degradation of extracellular matrix is a critical step in tumour invasion and metastasis. To examine the role of trypsin in tumour dissemination, we cloned two variants (S4 and R3 cells) from STKM-1, a trypsinogen 1-producing diffuse gastric cancer cell line. Western blot analysis with antitrypsin antibody showed that 26 and 24 kDa proteins were highly detected in S4 conditioned medium (CM) in comparison to R3 CM. In addition to the 26 and 24 kDa proteins, 25 and 23 kDa bands, which correspond to enterokinase-activated trypsin, were found only in S4 CM. When the CMs of the two clones were treated with enterokinase, the 25 and 23 kDa trypsin activities in S4 CM were effectively increased as compared with R3 CM. When the two clones were inoculated intraperitoneally (i.p.) into nude mice, S4 cells strongly invaded the liver, pancreas and peritoneum and killed the hosts more rapidly than R3 cells: the 50% survival time was 50 days for S4 and 82 days for R3 cells. These results suggest that trypsin production is associated with the invasive growth of STKM-1 gastric cancer cells. |
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Authors:
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Y Kato; Y Nagashima; N Koshikawa; Y Miyagi; H Yasumitsu; K Miyazaki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of cancer (Oxford, England : 1990) Volume: 34 ISSN: 0959-8049 ISO Abbreviation: Eur. J. Cancer Publication Date: 1998 Jun |
Date Detail:
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Created Date: 1998-12-15 Completed Date: 1998-12-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9005373 Medline TA: Eur J Cancer Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1117-23 Citation Subset: IM |
Affiliation:
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Department of Oral Biochemistry, Kanagawa Dental College, Yokosuka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Humans Mice Mice, Nude Neoplasm Proteins / metabolism* Phenotype RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction / methods Stomach Neoplasms / metabolism*, pathology Trypsin / biosynthesis* Trypsinogen / biosynthesis Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Neoplasm Proteins; 0/RNA, Messenger; 9002-08-8/Trypsinogen; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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