| Production of transgenic rats by ooplasmic injection of spermatogenic cells exposed to exogenous DNA: a preliminary study. | |
| | |
MedLine Citation:
|
PMID: 15293216 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The aim of the present study was to investigate the efficiencies of producing transgenic rats by the ooplasmic injection of sperm heads (intracytoplasmic sperm injection: ICSI) and elongating spermatids (elongating spermatid injection: ELSI) exposed to the EGFP DNA solution. A slightly lower proportion of ICSI oocytes using sperm heads exposed to a concentration of 0.5 microg/ml DNA solution for 1 min developed into offspring (13.3%, 48/361) when compared to that of oocytes injected with nontreated sperm heads (19.4%, 32/165). Eight ICSI offspring were found to be EGFP-carrying transgenic rats (16.7% per offspring; 2.2% per embryo). After a 1-min exposure of the elongating spermatids to 5 microg/ml of DNA solution, 8.8% (45/511) of the ELSI oocytes developed into offspring while 12.7% (22/173) of the ELSI oocytes using nontreated spermatids developed. Six ELSI offspring carried the EGFP DNA (13.3% per offspring; 1.2% per embryo). The conventional pronuclear microinjection of 5 microg/ml of DNA solution resulted in the higher production of offspring (29.7%, 104/350) and the birth of three transgenic rats (2.9% per offspring; 0.9% per embryo). Thus, sperm heads and elongating spermatids were practically useful as the vector of exogenous DNA if the DNA-exposed spermatogenic cells were microinseminated into rat oocytes. |
| | |
Authors:
|
Megumi Kato; Ayako Ishikawa; Ryosuke Kaneko; Takeshi Yagi; Shinichi Hochi; Masumi Hirabayashi |
Related Documents
:
|
1008256 - Dna synthesis in the oocyte of the mature mouse: a radioautographic study. 7039706 - Actin in human spermatozoa. 23643886 - Optimizing blood collection, transport and storage conditions for cell free dna increas... 8550286 - Are mitochondria inherited paternally in ascaris? 21706406 - Genotoxicity in atlantic killifish (fundulus heteroclitus) from a pah-contaminated supe... 21824916 - Phosphorylation of polynucleotide kinase/ phosphatase by dna-dependent protein kinase a... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Molecular reproduction and development Volume: 69 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 2004 Oct |
Date Detail:
|
Created Date: 2004-08-04 Completed Date: 2005-11-15 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: United States |
Other Details:
|
Languages: eng Pagination: 153-8 Citation Subset: IM |
Affiliation:
|
National Institute for Physiological Sciences, Okazaki, Aichi, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Genetically Modified / genetics* DNA / genetics* Embryo Culture Techniques Female Green Fluorescent Proteins / genetics Male Oocytes / cytology* Rats / genetics* Sperm Head / transplantation* Sperm Injections, Intracytoplasmic Spermatids / transplantation* |
| Chemical | |
Reg. No./Substance:
|
147336-22-9/Green Fluorescent Proteins; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Temporospatial expression of placental lactogen and prolactin-related protein-1 genes in the bovine ...
Next Document: Effects of bone morphogenetic protein-7 (BMP-7) on primordial follicular growth in the mouse ovary.