Document Detail


Production of transgenic mice expressing the goat H-FABP gene by intratesticular injection.
MedLine Citation:
PMID:  23192615     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The objective of this study was to explore the possibility of obtaining stable transgenic animals by intratesticular injection. The recombinant vector pEGFP-H-FABP expressing the goat heart-type fatty acid binding protein and green fluorescent protein was mixed with liposome complexes and randomly injected into the testes of mice. Testicular section, fluorescence, and DNA detection assays of mouse sperm were performed to determine the integration of foreign DNA. The results showed that foreign DNA was successfully expressed in the treated mice. Furthermore, the expression and function of the foreign gene were analyzed in F(1) generation and F(2) generation mice at different levels, with the positive rates of foreign gene transfer into the F(1) and F(2) generations being 4.0 and 30.23 %, respectively. These results strongly support testicular injection as an effective method of producing transgenic animals and indicate that foreign genes can be stably passed on to the offspring. This research has theoretical and practical implications for the improvement in the quality of laboratory animals and for gene therapy.
Authors:
Hao Chen; Yanhui Yin; Yani Zhang; Min Sun; Tingfeng Chen; Caiye Zhu; Wei Li; Bichun Li; Huilin Yang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-29
Journal Detail:
Title:  Molecular biology reports     Volume:  -     ISSN:  1573-4978     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, 215000, Jiangsu, China.
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