| Production of superoxide by human malignant melanoma cells. | |
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MedLine Citation:
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PMID: 9835450 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Metastasis is a complicated multi-step process involving interactions between tumour cells, the extracellular matrix and the vessel walls. Experimental observations suggest that leucocyte migration and function could be a suitable model in order to understand tumour cell dissemination. In the present report we show and quantify the production of free radicals by human malignant melanoma cells (St-ml12) by means of a spectrophotometrical method, using an enzyme immunoassay reader. Endothelial cells and activated polymorphonuclear leucocytes were used as controls. Melanoma cells without stimulants produced large amounts of superoxide anion at an increasing rate in relation to time, which could be inhibited by superoxide dismutase. Production of hydrogen peroxide was minimal. The endothelial cells produced a negligible amount, in contrast to the activated polymorphonuclear leucocytes, which released large quantities of both free radicals. A rapid assay to analyse the production of free radicals by tumour cells is presented here. Using this, we demonstrated that melanoma cells produce superoxide anions, supporting previous observations which implicate superoxide anion in the mechanism of metastasis. |
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Authors:
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F Bittinger; J L González-García; C L Klein; C Brochhausen; F Offner; C J Kirkpatrick |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Melanoma research Volume: 8 ISSN: 0960-8931 ISO Abbreviation: Melanoma Res. Publication Date: 1998 Oct |
Date Detail:
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Created Date: 1999-01-29 Completed Date: 1999-01-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9109623 Medline TA: Melanoma Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 381-7 Citation Subset: IM |
Affiliation:
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Institute of Pathology, Johannes Gutenberg-University, Mainz, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Humans Hydrogen Peroxide / metabolism Immunoenzyme Techniques Melanoma / blood supply, metabolism*, secondary Superoxides / metabolism* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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11062-77-4/Superoxides; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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