| Production and secretion of fatty acids in genetically engineered cyanobacteria. | |
| | |
MedLine Citation:
|
PMID: 20351245 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
Our purpose is to apply a fatty acid secretion strategy in photosynthetic microbial biofuel production, which will avoid the costly biomass recovery processes currently applied in algal biofuel systems. Starting with introducing acyl-acyl carrier protein thioesterases, we made five successive generations of genetic modifications into cyanobacterium Synechocystis sp. PCC 6803. The mutant strains were able to overproduce fatty acids (C10-C18) and secrete them into the medium at an efficiency of up to 133 +/- 12 mg/L of culture per day at a cell density of 1.5 x 10(8) cells/mL (0.23 g of dry weight/liter). Fatty acid secretion yields were increased by weakening the S layer and peptidoglycan layers. Although the fatty acid secreting strains had a long lag phase with many cells having damaged cell membranes when grown at low cell densities, these strains grew more rapidly in stationary phase and exhibited less cell damage than wild-type in a stationary culture. Our results suggest that fatty acid secreting cyanobacteria are a promising technology for renewable biofuel production. |
| | |
Authors:
|
Xinyao Liu; Daniel Brune; Wim Vermaas; Roy Curtiss |
Related Documents
:
|
7684285 - Cholinergic and pentagastrin stimulation of the gastric secretions of acid, pepsin and ... 8214065 - Gastrin cell responses to acidification of the achlorhydric rat stomach. 16494505 - Cvp-bias as an index to predict the life style of last common ancestor. 7844955 - Gastric acid secretion in the cynomolgus monkey. 11563375 - Ammonia inhibition in the anaerobic treatment of fishery effluents. 16406465 - Flavour formation by amino acid catabolism. |
Publication Detail:
|
Type: Journal Article; Retracted Publication Date: 2010-07-02 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: - ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-07-30 Completed Date: 2010-08-13 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: - Citation Subset: - |
Affiliation:
|
Center for Infectious Diseases and Vaccinology, The Biodesign Institute, and School of Life Sciences, Arizona State University, Tempe, AZ 85287-5401. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
Retraction In:
|
Schekman R. Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):13189
[PMID:
20616067
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Climate as a contributing factor in the demise of Angkor, Cambodia.
Next Document: Elongated oligomers in {beta}2-microglobulin amyloid assembly revealed by ion mobility spectrometry-...