| Production of replication-deficient adenovirus recombinants. | |
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MedLine Citation:
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PMID: 21374395 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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In essence, replication-deficient (RD) adenovirus (Ad) vectors can be considered to function as an extremely efficient DNA transfection system capable of providing transgene expression in up to 100% of cells both in vitro or in vivo. As researchers continue to realize the full potential of this vector system, discover novel applications, and further develop and enhance systems, the use of this vector system has increased exponentially. The exploitation of Ad recombinants in HCV research is encouraged by demonstration that the virus will efficiently infect and express transgenes in hepatocytes and that following iv inoculation, transgene expression can readily be detected in hepatocytes in the liver (1-5). A number of HCV proteins have now been expressed in Ad recombinants (6-8) whereas these vectors have also been used to deliver antisense and ribozyme molecules as prototype HCV therapeutics (9). |
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Authors:
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G W Wilkinson; C Rickards; B E Clarke |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular medicine Volume: 19 ISSN: 1543-1894 ISO Abbreviation: Methods Mol. Med. Publication Date: 1999 |
Date Detail:
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Created Date: 2011-03-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101123138 Medline TA: Methods Mol Med Country: United States |
Other Details:
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Languages: eng Pagination: 539-51 Citation Subset: - |
Affiliation:
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Department of Medicine, University of Wales College of Medicine, Cardiff, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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