| Production and release of plasminogen by isolated perfused rat liver. | |
| | |
MedLine Citation:
|
PMID: 6935686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In view of conflicting evidence for a major hepatic role in the synthesis of circulating plasminogen, the precursor of the fibrinolytic enzyme plasmin, we carried out the present study with a sensitive assay in order to measure the accumulation of small quantities of plasminogen in a recycling rat liver perfusion system. We have purified plasminogen from Sprague-Dawley rat plasma and have raised a monospecific antiserum against it in rabbits. Isolated rat liver perfusions were performed with an oxygenated recycling perfusate consisting of a perfluorotributylamine/Pluronic F-68 emulsion (Fluosol 43) free of plasma proteins and blood cells. The system was shown to be capable of synthesizing albumin and transferrin. The cumulative appearance of plasminogen in the perfusate was measured by a sensitive, specific radioimmunoassay. Plasminogen concentration increased progressively during the first 2 hr of perfusion; the observed average net synthesis in five separate experiments was approximately 35 microgram/hr per 100 g of body weight. Exposure of the perfused liver to 18 microM cycloheximide inhibited additional increase in the titer of plasminogen. Evidence for de novo synthesis was provided by the incorporation of 14C-labeled leucine into specific immunoprecipitates of plasminogen and the inhibition of this incorporation by cycloheximide. Analysis of the immunoprecipitates by sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed a single peak of radioactivity corresponding to Mr of 82,000. These data indicate that the liver is a major site of plasminogen production. |
| | |
Authors:
|
H Saito; S M Hamilton; A S Tavill; L Louis; O D Ratnoff |
Related Documents
:
|
7407256 - Uptake of asialoglycophorin-liposomes by the perfused rat liver. 2088746 - The effect of mono(2-ethylhexyl)phthalate on an isolated perfused rat heart-lung prepar... 3743526 - Evaluation of heparin toxicity in the isolated and perfused rat liver. 9881656 - Hepatic disposition characteristics of recombinant human interleukin-11 (rhil-11) in th... 8558296 - Purified ferritin and soybean meal can be sources of iron for treating iron deficiency ... 18085886 - Rat behavioral thermoregulation integrates with nonshivering thermogenesis during postn... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 77 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1980 Nov |
Date Detail:
|
Created Date: 1981-03-24 Completed Date: 1981-03-24 Revised Date: 2010-09-13 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 6837-40 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cycloheximide / pharmacology Liver / metabolism* Plasminogen / biosynthesis, metabolism*, secretion Rats |
| Grant Support | |
ID/Acronym/Agency:
|
HL 01661/HL/NHLBI NIH HHS; RR-05410-16/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
66-81-9/Cycloheximide; 9001-91-6/Plasminogen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Anti-gamete monoclonal antibodies synergistically block transmission of malaria by preventing fertil...
Next Document: Generation of human monoclonal antibodies reactive with human mammary carcinoma cells.