Document Detail


Production, recovery and purification of bacteriocins from lactic acid bacteria.
MedLine Citation:
PMID:  10570809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteriocins produced by lactic acid bacteria are a heterogeneous group of peptide inhibitors which include lantibiotics (class I, e.g. nisin), small heat-stable peptides (class II, e.g. pediocin AcH/PA1) and large heat-labile proteins (class III, e.g. helveticin J). Many bacteriocins belonging to the first two groups can be successfully used to inhibit undesirable microorganisms in foods, but only nisin is produced industrially and is licensed for use as a food preservative in a partially purified form. This review focuses on the production and purification of class I and class II bacteriocins from lactic acid bacteria. Bacteriocin production is growth associated but the yield of bacteriocin per unit biomass is affected by several factors, including the producing strain, media (carbohydrate and nitrogen sources, cations, etc.) and fermentation conditions (pH, temperature, agitation, aeration and dilution rate in continuous fermentations). Continuous fermentation processes with cell recycle or immobilized cells can result in a dramatic improvement in productivity over batch fermentations. Several simple recovery processes, based on adsorbing bacteriocin on resins or silica compounds, have been developed and can be used to build integrated production processes.
Authors:
E Parente; A Ricciardi
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  52     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-09     Completed Date:  1999-12-09     Revised Date:  2005-11-16    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  628-38     Citation Subset:  IM    
Affiliation:
Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università della Basilicata, Potenza, Italy. parente@unibas.it
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Amino Acid Sequence
Bacteriocins / genetics,  isolation & purification,  metabolism*
Culture Media / chemistry
Hydrogen-Ion Concentration
Lactobacillus / metabolism*
Molecular Sequence Data
Resins, Plant / metabolism
Silicon Dioxide / metabolism
Temperature
Chemical
Reg. No./Substance:
0/Bacteriocins; 0/Culture Media; 0/Resins, Plant; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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