| Production of reactive oxygen species in mitochondria of HeLa cells under oxidative stress. | |
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MedLine Citation:
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PMID: 16678116 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondria can be a source of reactive oxygen species (ROS) and a target of oxidative damage during oxidative stress. In this connection, the effect of photodynamic treatment (PDT) with Mitotracker Red (MR) as a mitochondria-targeted photosensitizer has been studied in HeLa cells. It is shown that MR produces both singlet oxygen and superoxide anion upon photoactivation and causes photoinactivation of gramicidin channels in a model system (planar lipid bilayer). Mitochondria-targeted antioxidant (MitoQ) inhibits this effect. In living cells, MR-mediated PDT initiates a delayed ("dark") accumulation of ROS, which is accelerated by inhibitors of the respiratory chain (piericidin, rotenone and myxothiazol) and inhibited by MitoQ and diphenyleneiodonium (an inhibitor of flavin enzymes), indicating that flavin of Complex I is involved in the ROS production. PDT causes necrosis that is prevented by MitoQ. Treatment of the cell with hydrogen peroxide causes accumulation of ROS, and the effects of inhibitors and MitoQ are similar to that described for the PDT model. Apoptosis caused by H2O2 is augmented by the inhibitors of respiration and suppressed by MitoQ. It is concluded that the initial segments of the respiratory chain can be an important source of ROS, which are targeted to mitochondria, determining the fate of the cell subjected to oxidative stress. |
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Authors:
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Boris V Chernyak; Denis S Izyumov; Konstantin G Lyamzaev; Alina A Pashkovskaya; Olga Y Pletjushkina; Yuri N Antonenko; Dmitrii V Sakharov; Karel W A Wirtz; Vladimir P Skulachev |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-04-07 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1757 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2006 May-Jun |
Date Detail:
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Created Date: 2006-07-10 Completed Date: 2006-09-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 525-34 Citation Subset: IM |
Affiliation:
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A. N. Belozersky Institute, Moscow State University, 119992 Moscow, Russia. bcheryak@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology Cell Death Cell Respiration Darkness Gramicidin / metabolism Hela Cells Humans Hydrogen Peroxide / pharmacology Light Lipid Bilayers / chemistry Methacrylates / pharmacology Mitochondria / drug effects, physiology*, radiation effects Onium Compounds / pharmacology Organophosphorus Compounds / pharmacology Oxidative Stress* Photochemotherapy Photosensitizing Agents / pharmacology Reactive Oxygen Species / metabolism* Rotenone / pharmacology Singlet Oxygen / metabolism Superoxides / metabolism Thiazoles / pharmacology Ubiquinone / analogs & derivatives, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/10-(6'-ubiquinonyl)decyltriphenylphosphonium bromide; 0/Antioxidants; 0/Lipid Bilayers; 0/Methacrylates; 0/Onium Compounds; 0/Organophosphorus Compounds; 0/Photosensitizing Agents; 0/Reactive Oxygen Species; 0/Thiazoles; 11062-77-4/Superoxides; 1339-63-5/Ubiquinone; 1405-97-6/Gramicidin; 17778-80-2/Singlet Oxygen; 244-54-2/diphenyleneiodonium; 76706-55-3/myxothiazol; 7722-84-1/Hydrogen Peroxide; 83-79-4/Rotenone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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