| Production of reactive oxygen species after photodynamic therapy by porphyrin sensitizers. | |
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MedLine Citation:
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PMID: 18645224 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objectives of this study was to investigate the production of reactive oxygen species (ROS) after photodynamic therapy (PDT) in vitro. We examined second generation sensitizers, porphyrines (TPPS4, ZnTPPS4 and PdTPPS4) and compared their effectivity on ROS generation in G361 cell line. Used porphyrines are very efficient water-soluble aromatic dyes with potential to use in photomedicine and have a high propensity to accumulate in the membranes of intracellular organelles like lysosomes and mitochondria. Interaction between the triplet excited state of the sensitizer and molecular oxygen leads to produce singlet oxygen and other ROS to induce cell death. Production of ROS was verificated by molecular probe CM-H2DCFDA and viability of cells was determined by MTT assay. Our results demonstrated that ZnTPPS4 induces the highest ROS production in cell line compared to TPPS4 and PdTPPS4 at each used concentration and light dose. These results consist with a fact that photodynamic effect depends on sensitizer type, its concentration and light dose. |
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Authors:
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H Kolarova; P Nevrelova; K Tomankova; P Kolar; R Bajgar; J Mosinger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: General physiology and biophysics Volume: 27 ISSN: 0231-5882 ISO Abbreviation: Gen. Physiol. Biophys. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-07-22 Completed Date: 2008-11-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8400604 Medline TA: Gen Physiol Biophys Country: Slovakia |
Other Details:
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Languages: eng Pagination: 101-5 Citation Subset: IM |
Affiliation:
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Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacký University, Hnevotínská 3, 775 15 Olomouc, Czech Republic. kol@tunw.upol.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Humans Melanoma Palladium* Photochemotherapy* Photosensitizing Agents / pharmacology* Porphyrins / pharmacology* Reactive Oxygen Species / metabolism* Zinc* |
| Chemical | |
Reg. No./Substance:
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0/Photosensitizing Agents; 0/Porphyrins; 0/Reactive Oxygen Species; 0/meso-tetrakis(4-sulfonatophenyl)porphyrin; 7440-05-3/Palladium; 7440-66-6/Zinc |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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