Document Detail


Production of a rat T cell hybridoma that stably expresses the T cell differentiation marker RT6.2.
MedLine Citation:
PMID:  3262567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rat T cell alloantigen RT6.2 shows a slow rate of synthesis in isolated T cells which hampers studies on the metabolism of RT6.2 in these cells (1). In order to facilitate further molecular and functional characterization of this molecule we have established a T-T hybridoma cell line which stably expresses RT6.2. Only 1 of 102 T cell hybridomas obtained upon fusion of the rat thymoma C58NT with DA rat lymph node cells expressed this antigen. This clone--EpD3--initially showed a relatively slow rate of cell division and a highly unstable pattern of expression of RT6.2. Thus, the relative number of RT6.2 bearing cells consistently decreased during cultivation of EpD3 and of EpD3-derived subclones. Cell separation studies suggest that the switch in RT6.2 phenotype of EpD3 cultures was due to the appearance of variant cells of RT6.2- phenotype with a higher proliferative capacity and was not induced by factors in the culture medium. By repeated panning and subcloning procedures to select for RT6.2 expressing cells, a subline of EpD3--EpD3/87--was obtained which stably expresses high levels of RT6.2. Metabolic labeling studies of RT6.2 in EpD3 show that it is synthesized very efficiently in these cells and support our previous suggestion that RT6.2 does not bear any classic oligosaccharide side chains. Moreover, RT6.2 can be released almost completely from EpD3 cells by phosphatidylinositol-specific phospholipase C (PI-PLC).
Authors:
F Koch; A Kashan; H G Thiele
Related Documents :
15341747 - Eff-1 is sufficient to initiate and execute tissue-specific cell fusion in c. elegans.
18059527 - Expression of fusion il2-b7.1(igv+c) and effects on t lymphocytes.
6731437 - Complementation of multiple sulfatase deficiency in somatic cell hybrids.
565787 - Transfer of human chromosomes via human minisegregant cells into mouse cells and the qu...
7738787 - Thermally-induced cell lysis in escherichia coli k12.
24424767 - A structural glycoprotein, containing hydroxyproline, isolated from the cell wall of ch...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hybridoma     Volume:  7     ISSN:  0272-457X     ISO Abbreviation:  Hybridoma     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-11-23     Completed Date:  1988-11-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8202424     Medline TA:  Hybridoma     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  341-53     Citation Subset:  IM    
Affiliation:
Department of Immunology, University Hospital, Hamburg, Federal Republic of Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Differentiation, T-Lymphocyte / analysis*
Biological Markers / analysis
DNA / metabolism
Hybridomas / immunology*,  metabolism
Mitogens / pharmacology
Rats
T-Lymphocytes / immunology*,  metabolism
Chemical
Reg. No./Substance:
0/Antigens, Differentiation, T-Lymphocyte; 0/Biological Markers; 0/Mitogens; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Clinical effects of monoclonal antibodies (MAb 17-1A) in patients with metastatic colorectal carcino...
Next Document:  Further characterization of the fate of human monoclonal antibodies in rhesus monkeys.