| Production, purification and characterization of an anti-(carcinoembryonic antigen) recombinant single-chain Fv antibody fragment. | |
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MedLine Citation:
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PMID: 8756397 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Specific targeting of radioactive agents to tumour cells has been the main objective of the in vivo use of monoclonal antibodies and their fragments. In particular, specific antibodies to carcinoembryonic antigen (CEA)-expressing tumours can be used either for diagnosis or therapy, if targeting could be improved. The expression of antibody fragments in bacteria allows the preparation of engineered molecules with antigen-binding properties and a better penetration into the tumour. A specific anti-CEA single-chain Fv fragment was produced in bacteria and purified. Its binding activity has been demonstrated in ELISA, immunocytochemistry, immunohistochemistry, fluorescence-activated cell sorting and the kinetic parameters determined by the plasmon surface resonance. |
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Authors:
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L Pérez; J E Vázquez; M Ayala; A G Siccardi; E Tresoldi; C de Lalla; J V Gavilondo; A Sidoli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and applied biochemistry Volume: 24 ( Pt 1) ISSN: 0885-4513 ISO Abbreviation: Biotechnol. Appl. Biochem. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1996-09-20 Completed Date: 1996-09-20 Revised Date: 2007-03-21 |
Medline Journal Info:
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Nlm Unique ID: 8609465 Medline TA: Biotechnol Appl Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 79-82 Citation Subset: IM |
Affiliation:
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Department of Biological and Technological Research, San Raffaele Scientific Institute, Milano, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Neoplasm
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biosynthesis*,
genetics,
isolation & purification Base Sequence Biotechnology Carcinoembryonic Antigen / immunology* Cell Line DNA Primers / genetics Escherichia coli / genetics Genetic Vectors Humans Immunoglobulin Fragments / biosynthesis*, genetics, isolation & purification Immunohistochemistry Kinetics Molecular Sequence Data Recombinant Proteins / biosynthesis, genetics, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Neoplasm; 0/Carcinoembryonic Antigen; 0/DNA Primers; 0/Immunoglobulin Fragments; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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