Document Detail

Production of proteinase A by Saccharomyces cerevisiae in a cell-recycling fermentation system: experiments and computer simulations.
MedLine Citation:
PMID:  8867629     Owner:  NLM     Status:  MEDLINE    
Overproduction of proteinase A by recombinant Saccharomyces cerevisiae was investigated by cultivations in a cell-recycling bioreactor. Membrane filtration was used to separate cells from the broth. Recycling ratios and dilution rates were varied and the effect on enzyme production was studied both experimentally and by computer simulations. Experiments and simulations showed that cell mass and product concentration were enhanced by high ratios of recycling. Additional simulations showed that the proteinase A concentration decreased drastically at high dilution rates and the optimal volumetric productivities were at high dilution rates just below washout and at high ratios of recycling. Cell-recycling fermentation gave much higher volumetric productivities and stable product concentrations in contrast to simple continuous fermentation.
S Grøn; K Biedermann; C Emborg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  44     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-11-19     Completed Date:  1996-11-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  724-30     Citation Subset:  B    
Department of Biotechnology, Technical University of Denmark, Lyngby.
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MeSH Terms
Aspartic Acid Endopeptidases / biosynthesis*
Computer Simulation
Models, Biological
Saccharomyces cerevisiae / metabolism*
Reg. No./Substance:
EC 3.4.23.-/Aspartic Acid Endopeptidases; EC II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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