| Production of proteinase A by Saccharomyces cerevisiae in a cell-recycling fermentation system: experiments and computer simulations. | |
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MedLine Citation:
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PMID: 8867629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Overproduction of proteinase A by recombinant Saccharomyces cerevisiae was investigated by cultivations in a cell-recycling bioreactor. Membrane filtration was used to separate cells from the broth. Recycling ratios and dilution rates were varied and the effect on enzyme production was studied both experimentally and by computer simulations. Experiments and simulations showed that cell mass and product concentration were enhanced by high ratios of recycling. Additional simulations showed that the proteinase A concentration decreased drastically at high dilution rates and the optimal volumetric productivities were at high dilution rates just below washout and at high ratios of recycling. Cell-recycling fermentation gave much higher volumetric productivities and stable product concentrations in contrast to simple continuous fermentation. |
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Authors:
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S Grøn; K Biedermann; C Emborg |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 44 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-11-19 Completed Date: 1996-11-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 724-30 Citation Subset: B |
Affiliation:
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Department of Biotechnology, Technical University of Denmark, Lyngby. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspartic Acid Endopeptidases
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biosynthesis* Bioreactors* Computer Simulation Fermentation* Models, Biological Saccharomyces cerevisiae / metabolism* |
| Chemical | |
Reg. No./Substance:
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EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.23.19/aspergillopepsin II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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