| Production of procollagenase by cultured human keratinocytes. | |
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MedLine Citation:
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PMID: 2433269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using a collagen film assay utilizing 14C-labeled type I collagen, we demonstrated that cultured human keratinocytes produced a procollagenase after treatment with the tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA). Production of collagenase paralleled alterations in cellular morphology induced by TPA. When procollagenase was immunoprecipitated with antibody to human fibroblast collagenase and analyzed on sodium dodecyl sulfate-polyacrylamide gels, the zymogen was revealed as a 56- and 51-kDa doublet. The keratinocyte-derived collagenase was a neutral metalloprotease, required activation with trypsin for detection in the collagenase assay and produced the characteristic three-quarter and one-quarter length collagen cleavage products when incubated with type I collagen at 25 degrees C. The enzyme was inhibited by serum and cysteine and was largely unaffected by serine, thiol, and carboxyl protease inhibitors. Cycloheximide inhibited the TPA-induced production of collagenase, suggesting that the procollagenase was not stored preformed in the keratinocytes. Keratinocytes treated with a tumor-promoting analogue of TPA also produced collagenase, but cells treated with cytochalasin B, interleukin-1, or two non-tumor promoting phorbol esters did not. Keratinocyte-derived collagenase may play a role in wound healing and morphogenesis. |
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Authors:
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M J Petersen; D T Woodley; G P Stricklin; E J O'Keefe |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 262 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1987 Jan |
Date Detail:
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Created Date: 1987-02-25 Completed Date: 1987-02-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 835-40 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aging Cells, Cultured Collagenases* Enzyme Precursors / biosynthesis*, metabolism Fluorescent Antibody Technique Humans Infant, Newborn Keratins / analysis* Kinetics Male Microbial Collagenase / biosynthesis*, metabolism Skin / enzymology*, growth & development Tetradecanoylphorbol Acetate / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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AM 00977/AM/NIADDK NIH HHS; AM 25871/AM/NIADDK NIH HHS; AM 33625/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Precursors; 16561-29-8/Tetradecanoylphorbol Acetate; 68238-35-7/Keratins; EC 3.4.24.-/Collagenases; EC 3.4.24.-/procollagenase; EC 3.4.24.3/Microbial Collagenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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