Document Detail


Production of polyesters consisting of medium chain length 3-hydroxyalkanoic acids by Pseudomonas mendocina 0806 from various carbon sources.
MedLine Citation:
PMID:  10696875     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pseudomonas mendocina strain 0806 was isolated from oil-contaminated soil and found to produce polyesters consisting of medium chain length 3-hydroxyalkanoates (mclPHAs). The monomers of mclPHAs contained even numbers of carbon atoms, such as 3-hydroxyhexanoate (HHx or C6), 3-hydroxyoctanoate (HO or C8), and/or 3-hydroxydecanoate (HD or C10) as major components when grown on many carbon sources unrelated to their monomeric structures, such as glucose, citric acid, and carbon sources related to their monomeric structures, such as myristic acid, octanoate, or oleic acid. On the other hand, PHA containing both even and odd numbers of hydroxyalkanoates (HA) monomers was synthesized when the strain was grown on tridecanoic acid. The molar ratio of carbon to nitrogen (C/N) had a significant effect on PHA composition: the strain produced PHAs containing 97-99% of HD monomer when grown in a glucose ammonium sulfate medium of C/N<20, and 20% HO, and 80% of the HD monomer when growth was conducted in media containing C/N>40. It was demonstrated that the HO/HD ratio in the polymers remained constant in media with a constant C/N ratio, regardless of the glucose concentration. Up to 3.6 g/L cell dry weight containing 45% of PHAs was produced when the strain was grown for 48 h in a medium containing 20 g/L glucose with a C/N ratio of 40.
Authors:
W Tian; K Hong; G Q Chen; Q Wu; R Q Zhang; W Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Antonie van Leeuwenhoek     Volume:  77     ISSN:  0003-6072     ISO Abbreviation:  Antonie Van Leeuwenhoek     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-04-14     Completed Date:  2000-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372625     Medline TA:  Antonie Van Leeuwenhoek     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  31-6     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Glucose / metabolism
Hydroxy Acids / metabolism*
Petroleum
Polyesters / metabolism*
Pseudomonas / isolation & purification,  metabolism*
Soil Microbiology*
Soil Pollutants
Chemical
Reg. No./Substance:
0/Hydroxy Acids; 0/Petroleum; 0/Polyesters; 0/Soil Pollutants; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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