| Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration. | |
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MedLine Citation:
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PMID: 23154468 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Although phosphatidylinositol 5-phosphate (PtdIns5P) is present in many cell types and its biogenesis is increased by diverse stimuli, its precise cellular function remains elusive. Here we show that PtdIns5P levels increase when cells are stimulated to move and we find PtdIns5P to promote cell migration in tissue culture and in a Drosophila in vivo model. First, class III phosphatidylinositol 3-kinase, which produces PtdIns3P, was shown to be involved in migration of fibroblasts. In a cell migration screen for proteins containing PtdIns3P-binding motifs, we identified the phosphoinositide 5-kinase PIKfyve and the phosphoinositide 3-phosphatase MTMR3, which together constitute a phosphoinositide loop that produces PtdIns5P via PtdIns(3,5)P(2). The ability of PtdIns5P to stimulate cell migration was demonstrated directly with exogenous PtdIns5P and a PtdIns5P-producing bacterial enzyme. Thus, the identified phosphoinositide loop defines a new role for PtdIns5P in cell migration. |
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Authors:
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Angela Oppelt; Viola H Lobert; Kaisa Haglund; Ashley M Mackey; Lucia E Rameh; Knut Liestøl; Kay Oliver Schink; Nina Marie Pedersen; Eva M Wenzel; Ellen M Haugsten; Andreas Brech; Tor Erik Rusten; Harald Stenmark; Jørgen Wesche |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-16 |
Journal Detail:
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Title: EMBO reports Volume: - ISSN: 1469-3178 ISO Abbreviation: EMBO Rep. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100963049 Medline TA: EMBO Rep Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1] Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo [2] Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Montebello, N-0310 Oslo. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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