| Production of a novel polyketide through the construction of a hybrid polyketide synthase. | |
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MedLine Citation:
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PMID: 8996112 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lactone rings of the polyketides platenolide and tylactone are synthesized by condensation of acetate-, proprionate-, and butyrate-derived precursors. A hybrid tylactone/platenolide synthase was constructed to determine if the choice of substrate is programmed by the polyketide synthase and to ascertain if a substrate different than that normally used in the first step of platenolide synthesis could be incorporated into the final polyketide. In this work, we report the successful incorporation of a propionate in place of the acetate normally used in the first step of platenolide synthesis. This result demonstrates that polyketide synthases choose a particular substrate at defined steps and provides strong evidence that substrate choice is programmed by the acyl transferase domain of a large, multifunctional polyketide synthase. |
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Authors:
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S Kuhstoss; M Huber; J R Turner; J W Paschal; R N Rao |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Gene Volume: 183 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-02-13 Completed Date: 1997-02-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 231-6 Citation Subset: IM |
Affiliation:
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Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acyl Coenzyme A
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metabolism Acyltransferases / metabolism Anti-Bacterial Agents / biosynthesis Lactones / metabolism* Macrolides Malonyl Coenzyme A / metabolism Multienzyme Complexes / genetics, metabolism* Open Reading Frames / genetics Recombinant Proteins / genetics Sequence Deletion Streptomyces / enzymology*, genetics Substrate Specificity Tylosin / analogs & derivatives, biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/16-methylplatenolide I; 0/Acyl Coenzyme A; 0/Anti-Bacterial Agents; 0/Lactones; 0/Macrolides; 0/Multienzyme Complexes; 0/Recombinant Proteins; 1401-69-0/Tylosin; 317-66-8/propionyl-coenzyme A; 52212-94-9/platenolide I; 524-14-1/Malonyl Coenzyme A; 74758-60-4/protylonolide; EC 2.3.-/Acyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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