Document Detail

Production of a novel polygalacturonic acid bioflocculant REA-11 by Corynebacterium glutamicum.
MedLine Citation:
PMID:  15081493     Owner:  NLM     Status:  MEDLINE    
The production of a novel polygalacturonic acid bioflocculant REA-11 from a newly isolated strain, Corynebacterium glutamicum CCTCC M201005, was investigated. Sucrose was chosen as a carbon source for REA-11 production. Complex nitrogen sources containing urea and an organic nitrogen compound enhanced both bacterial growth and REA-11 production, among which urea plus corn steep liquor was shown to be the most efficient combination. A cost-effective medium for REA-11 production mainly comprised 17 g/l sucrose, 0.45 g/l urea, and 5 ml/l corn steep liquor, under which conditions the flocculating activity reached 390 U/ml. The molar ratio of carbon to nitrogen (C/N) significantly affected REA-11 production, where a C/N ratio of 20:1 was shown to be the best. Interestingly, by simultaneously feeding sucrose and urea at a C/N ratio of 20:1 at 24 h of fermentation, REA-11 production (458 U/ml) was enhanced by 17% compared to the control. In a 10 l jar fermentor, lower dissolved oxygen tension was favorable for REA-11 production: a flocculating activity of 520 U/ml was achieved at a kappaLa of 100 h(-1). REA-11 raw product is relatively thermo-stable at acidic pH ranges of 3.0-6.5. Preliminary application studies showed that REA-11 had stronger flocculating activity to Kaolin clay suspension compared to chemical flocculants. In addition, the capability of decolorizing molasses wastewater indicates the industrial potential of this novel bioflocculant.
Ning He; Yin Li; Jian Chen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioresource technology     Volume:  94     ISSN:  0960-8524     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-04-14     Completed Date:  2004-08-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  99-105     Citation Subset:  IM    
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi, PR China.
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MeSH Terms
Carbon / analysis
Corynebacterium / metabolism*
Hydrogen-Ion Concentration
Nitrogen / analysis
Pectins / metabolism*
Waste Disposal, Fluid / methods*
Zea mays / chemistry
Reg. No./Substance:
0/Pectins; 57-13-6/Urea; 57-50-1/Sucrose; 7440-44-0/Carbon; 7727-37-9/Nitrogen; 9046-38-2/polygalacturonic acid

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