Document Detail


Production of a new D-amino acid oxidase from the fungus Fusarium oxysporum.
MedLine Citation:
PMID:  10427080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives as inducers. The best inducers were D-alanine (2.7 microkat/g of dry biomass) and D-3-aminobutyric acid (2.6 microkat/g of dry biomass). The addition of zinc ions was necessary to permit the induction of peroxisomal D-amino acid oxidase. Bioreactor cultivations were performed on a 50-liter scale, yielding a volumetric D-amino acid oxidase activity of 17 microkat liter(-1) with D-alanine as an inducer. Under oxygen limitation, the volumetric activity was increased threefold to 54 microkat liter(-1) (3,240 U liter(-1)).
Authors:
M Gabler; L Fischer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  65     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-15     Completed Date:  1999-09-15     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3750-3     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, 38106 Braunschweig, Germany.
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MeSH Terms
Descriptor/Qualifier:
Bioreactors
Biotechnology
D-Amino-Acid Oxidase / biosynthesis*
Fusarium / enzymology*
Oxygen
Chemical
Reg. No./Substance:
7782-44-7/Oxygen; EC 1.4.3.3/D-Amino-Acid Oxidase
Comments/Corrections

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