| Production of mouse chimeras by injection of embryonic stem cells into the perivitelline space of one-cell stage embryos. | |
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MedLine Citation:
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PMID: 20143261 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Generation of mouse chimeras is useful for the elucidation of gene function. In the present report, we describe a new technique for the production of chimeras by injection of R1 embryonic stem (ES) cells into the perivitelline space of one-cell stage mouse embryos. One-cell embryos are injected with 2-6 ES cells into the perivitelline space under the zona pellucida without laser-assistance. Our embryo culture experiments reveal that ES cells injected at the one-cell stage embryo start to be incorporated into the blastomeres beginning at the 8-cell stage and form a chimeric blastocyst after 4 days. We have used this approach to successfully produce a high rate of mouse chimeras in two different mouse genetic backgrounds permitting the establishment of germ line transmitters. This method allows for the earlier introduction of ES cells into mouse embryos, and should free up the possibility of using frozen one-cell embryos for this purpose. |
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Authors:
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Yves De Repentigny; Rashmi Kothary |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-09 |
Journal Detail:
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Title: Transgenic research Volume: 19 ISSN: 1573-9368 ISO Abbreviation: Transgenic Res. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-03 Completed Date: 2011-02-24 Revised Date: 2011-04-28 |
Medline Journal Info:
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Nlm Unique ID: 9209120 Medline TA: Transgenic Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1137-44 Citation Subset: IM |
Affiliation:
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Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, K1H 8L6, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / cytology, metabolism Cell Line Cell Transplantation / methods Cleavage Stage, Ovum / cytology*, metabolism Embryonic Stem Cells / transplantation* Female Germ Cells / cytology, metabolism Luminescent Proteins / genetics, metabolism Male Mice Mice, Transgenic Microinjections Microscopy, Fluorescence Pregnancy Recombinant Proteins / genetics, metabolism Transplantation Chimera* / genetics |
| Grant Support | |
ID/Acronym/Agency:
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//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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