Document Detail

Production of the monoterpene limonene and modulation of apoptosis-related proteins in embryonic-mouse NIH 3T3 fibroblast cells by introduction of the limonene synthase gene isolated from Japanese catnip (Schizonepeta tenuifolia).
MedLine Citation:
PMID:  18547169     Owner:  NLM     Status:  MEDLINE    
The monoterpene D-limonene shows cancer preventative and cancer therapeutic activities in vitro and in vivo. Unlike plants, animals are unable to synthesize limonene de novo and obtain limonene through dietary sources. In the present study we established transgenic mouse embryonic NIH 3T3 fibroblast cells that produce limonene by introducing the D-limonene synthase gene obtained from Japanese catnip (Schizonepeta tenuifolia). Apoptosis was not observed in the limonene-producing cells. A concomitant increase in the level of apoptosis-related protein Bcl-2 (B-cell lymphoma protein 2) and decreases in the levels of Bad (Bcl-2 antagonist of cell death) and phosphorylated JNK (c-Jun N-terminal kinase) were observed in limonene-producing cells. Limonene-producing cells may provide a useful new system to investigate the in vivo function of this monoterpene.
Yoshiko Satomi; Kazuaki Ohara; Kazufumi Yazaki; Michiho Ito; Gisho Honda; Hoyoku Nishino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  52     ISSN:  1470-8744     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-06     Completed Date:  2009-07-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  185-90     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, Japan.
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MeSH Terms
Apoptosis Regulatory Proteins / genetics,  metabolism*
Cell Cycle
Cell Proliferation
Cloning, Molecular
Cyclohexenes / metabolism*
Flow Cytometry
Gas Chromatography-Mass Spectrometry
Intramolecular Lyases / genetics,  metabolism*
JNK Mitogen-Activated Protein Kinases / genetics,  metabolism
Lamiaceae / enzymology*
Mice, Transgenic
NIH 3T3 Cells
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism
Terpenes / metabolism*
bcl-Associated Death Protein / genetics,  metabolism
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Bad protein, mouse; 0/Cyclohexenes; 0/Proto-Oncogene Proteins c-bcl-2; 0/Terpenes; 0/bcl-Associated Death Protein; 138-86-3/limonene; EC Mitogen-Activated Protein Kinases; EC 5.5.-/Intramolecular Lyases; EC 5.5.-/pinene cyclase I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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