Document Detail


Production of a monoclonal antibody to Vibrio cholerae non-O1 heat-stable enterotoxin (ST) which is cross-reactive with Yersinia enterocolitica ST.
MedLine Citation:
PMID:  1696928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A monoclonal antibody (MAb) against synthetic heat-stable enterotoxin of Vibrio cholerae non-O1 (NAG-ST) was produced. The MAb, namely, 2F, belonged to the immunoglobulin G1 class. Ascitic fluid drawn from pristane-primed BALB/c mice injected with a 2F-producing clone demonstrated anti-NAG-ST activity which could be detected in enzyme-linked immunosorbent assay even at a dilution of 1:128,000. Fifty-fold-diluted ascitic fluid could completely neutralize the activity of NAG-ST (synthetic and native) and Vibrio mimicus ST (identical to NAG-ST) in suckling mice. In the same assay, 2F could also neutralize Yersinia enterocolitica ST (Y-ST) but did not neutralize Escherichia coli STh and STp. A similar pattern of reactivity occurred in a competitive enzyme-linked immunosorbent assay with homologous and heterologous toxins. Competitive inhibition curves with synthetic peptides representing NAG-ST and its shorter analogs revealed that aspartic acid located at position 2 from the N terminus of NAG-ST was the essential residue of the recognized epitope. Significantly, in Y-ST, to which 2F cross-reacted, aspartic acid is in the corresponding position as that of NAG-ST, thereby confirming our conclusions that the epitope defining this MAb is aspartic acid.
Authors:
T Takeda; G B Nair; K Suzuki; Y Shimonishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  58     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-09-27     Completed Date:  1990-09-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2755-9     Citation Subset:  IM    
Affiliation:
Department of Infectious Diseases Research, National Children's Medical Research Center, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal / biosynthesis*
Cross Reactions
Enterotoxins / analysis*
Enzyme-Linked Immunosorbent Assay
Epitopes / immunology
Male
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Neutralization Tests
Vibrio cholerae / immunology*
Yersinia enterocolitica / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Enterotoxins; 0/Epitopes; 0/stN protein, Vibrio cholerae
Comments/Corrections

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