| Production of a monoclonal antibody to Vibrio cholerae non-O1 heat-stable enterotoxin (ST) which is cross-reactive with Yersinia enterocolitica ST. | |
| | |
MedLine Citation:
|
PMID: 1696928 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A monoclonal antibody (MAb) against synthetic heat-stable enterotoxin of Vibrio cholerae non-O1 (NAG-ST) was produced. The MAb, namely, 2F, belonged to the immunoglobulin G1 class. Ascitic fluid drawn from pristane-primed BALB/c mice injected with a 2F-producing clone demonstrated anti-NAG-ST activity which could be detected in enzyme-linked immunosorbent assay even at a dilution of 1:128,000. Fifty-fold-diluted ascitic fluid could completely neutralize the activity of NAG-ST (synthetic and native) and Vibrio mimicus ST (identical to NAG-ST) in suckling mice. In the same assay, 2F could also neutralize Yersinia enterocolitica ST (Y-ST) but did not neutralize Escherichia coli STh and STp. A similar pattern of reactivity occurred in a competitive enzyme-linked immunosorbent assay with homologous and heterologous toxins. Competitive inhibition curves with synthetic peptides representing NAG-ST and its shorter analogs revealed that aspartic acid located at position 2 from the N terminus of NAG-ST was the essential residue of the recognized epitope. Significantly, in Y-ST, to which 2F cross-reacted, aspartic acid is in the corresponding position as that of NAG-ST, thereby confirming our conclusions that the epitope defining this MAb is aspartic acid. |
| | |
Authors:
|
T Takeda; G B Nair; K Suzuki; Y Shimonishi |
Related Documents
:
|
16412708 - Determination of free amino acids and related compounds in amniotic fluid by capillary ... 9206098 - Protective effect of interleukin-1 receptor antagonist on oleic acid-induced lung injury. 6437358 - Hyperammonemic encephalopathy due to essential amino acid hyperalimentation. 8412018 - L-2-hydroxyglutaric acidaemia: clinical and biochemical findings in 12 patients and pre... 15876528 - Steady-state haemoglobin level in sickle cell anaemia increases with an increase in ery... 21327498 - Changes in digestive enzyme activity during initial ontogeny of bay snook petenia splen... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Infection and immunity Volume: 58 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1990 Sep |
Date Detail:
|
Created Date: 1990-09-27 Completed Date: 1990-09-27 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 2755-9 Citation Subset: IM |
Affiliation:
|
Department of Infectious Diseases Research, National Children's Medical Research Center, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Antibodies, Monoclonal / biosynthesis* Cross Reactions Enterotoxins / analysis* Enzyme-Linked Immunosorbent Assay Epitopes / immunology Male Mice Mice, Inbred BALB C Molecular Sequence Data Neutralization Tests Vibrio cholerae / immunology* Yersinia enterocolitica / immunology* |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Enterotoxins; 0/Epitopes; 0/stN protein, Vibrio cholerae |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mapping and conservation of the group-specific component gene in mouse.
Next Document: Characterization of rat T helper cell clones specific for Bacteroides gingivalis antigen.