Document Detail


Production of a monoclonal antibody reacted broadly with feline calicivirus field isolates.
MedLine Citation:
PMID:  9524937     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A monoclonal antibody (MAb) reactive with 36 field isolates and 2 laboratory strains of feline calicivirus (FCV) was produced by immunizing mice with the mixture of FCVs. The MAb (4D7) reacted with FCVs in an enzyme-linked immunosorbent assay (ELISA), but had no neutralizing activity against the F4 strain of FCV. MAb 8G1, previously produced against the FCV F4 strain, also reacted in ELISA with all FCVs used in the present study. However, the epitopes recognized by 4D7 and 8G1 were different. Using these two MAbs and a polyclonal rabbit antibody, we attempted to develop a sandwich ELISA for detection of FCV antigen. The combination of 4D7 and the polyclonal rabbit IgG was most sensitive. Using this system, all the field isolates of FCV cultured in vitro were detected. However, among the 36 swab samples, from which FCV was isolated, 4 were negative.
Authors:
T Tajima; S Yoshizaki; E Nakata; Y Tohya; S Ishiguro; Y Fujikawa; S Sugii
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  60     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-05-13     Completed Date:  1998-05-13     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  155-60     Citation Subset:  IM    
Affiliation:
Department of Veterinary Microbiology, College of Agriculture, Osaka Prefecture University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies
Antibodies, Monoclonal*
Antigen-Antibody Reactions
Antigens, Viral / analysis
Caliciviridae Infections / diagnosis,  veterinary*
Calicivirus, Feline / immunology*,  isolation & purification*
Cat Diseases*
Cats
Cell Line
Enzyme-Linked Immunosorbent Assay / methods
Immunoblotting
Immunoglobulin G
Kidney
Mice
Rabbits
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antibodies, Monoclonal; 0/Antigens, Viral; 0/Immunoglobulin G

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