| Production of monoclonal antibodies to preneoplastic liver cell populations induced by chemical carcinogens in rats and to transplantable Morris hepatomas. | |
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MedLine Citation:
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PMID: 2474376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monoclonal antibodies (moabs) to neoplastic and preneoplastic liver cells in rats have been selected to follow cellular changes in the livers during chemical carcinogenesis. The moabs were induced by immunizations of BALB/c mice with four partially purified liver cell preparations: 1) oval cells induced in male Fischer rats fed 0.05% N-2-acetylaminofluorene in a choline deficient diet: 2) preneoplastic gamma-glutamyltranspeptidase positive hepatocytes induced by i.p. injection of diethylnitrosamine into male Fischer rats followed by 0.02% N-2-acetylaminofluorene and partial hepatectomy (Solt-Farber model): 3) sharply dissected neoplastic nodules induced in male Fischer rats by five 2-week cycles of 0.05% N-2-acetylaminofluorene diet: and 4) Morris hepatomas 7777 and 5123 passaged in male Buffalo rats. The hybridomas were screened by enzyme linked immunosorbent assay or by indirect immunofluorescence on composite cryostat sections of fetal and adult rat liver, liver containing neoplastic nodules, and Morris hepatoma 7777. Positive clones were limit diluted and partially characterized by indirect immunofluorescence on cryostat sections of other preneoplastic and neoplastic rat livers as well as normal rat tissues. Two moabs to oval cells, two moabs to hepatocytes, and one moab to hepatomas have been selected for further study. |
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Authors:
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H A Dunsford; S Sell |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 49 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1989-09-15 Completed Date: 1989-09-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4887-93 Citation Subset: IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston 77225. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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2-Acetylaminofluorene Animals Antibodies, Monoclonal / biosynthesis*, immunology Antigens, Neoplasm / immunology Diethylnitrosamine Enzyme-Linked Immunosorbent Assay Epitopes / immunology Fetus / immunology Fluorescent Antibody Technique Hybridomas / immunology Liver / immunology Liver Neoplasms, Experimental / chemically induced, immunology* Male Mice Mice, Inbred BALB C Neoplasm Transplantation Precancerous Conditions / chemically induced, immunology* Rats Rats, Inbred F344 gamma-Glutamyltransferase / analysis |
| Grant Support | |
ID/Acronym/Agency:
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2 RO1 CA234635/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/Epitopes; 53-96-3/2-Acetylaminofluorene; 55-18-5/Diethylnitrosamine; EC 2.3.2.2/gamma-Glutamyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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