Document Detail


Production of monoclonal antibodies against Neisseria meningitidis using popliteal lymph nodes and in vivo/in vitro immunization: prevalence study of new monoclonal antibodies in greater São Paulo, Brazil.
MedLine Citation:
PMID:  17979546     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and efficient method for preparing monoclonal antibody (MAb) serotypes using Neisseria meningitidis outer membrane were used in BALB/c mouse footpads for the immunization. The popliteal lymph nodes were isolated 19 days later for MAb-producing hybridomas, from which the MAbs against the 37 kDa protein were screened. Variations in class 2/3 (PorB) proteins form the basis for meningococcal serotyping. This is the first report on the preparation of MAbs against N. meningitidis that is specific to PorB protein using popliteal lymph nodes. The new monoclonal antibodies were specific for PorB outer membrane protein FL24(PL)Br, a new serotype 24 class 3 antigens of non-typeable (NT:NST) serogroup B strain, and FL14(PL)Br specific for the serotype 14, and reacted with the S3446 reference strain analyzed. A total of 12% of the case isolates reacted with one or more of the monoclonal antibodies. The high-affinity MAbs produced by hybridoma methodology provide a basis for further research on the pathogenesis and early diagnosis of meningococcus.
Authors:
Elza F T Belo; Aline S Ferraz; Ligia M C C Coutinho; Ana P Oliveira; Andréia M S Carmo; Claudia F Tunes; Tatiane Ferreira; Andre Y Ito; Marta S F Machado; Daniele De L Franco; Elizabeth N De Gaspari
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hybridoma (2005)     Volume:  26     ISSN:  1554-0014     ISO Abbreviation:  Hybridoma (Larchmt)     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-11-05     Completed Date:  2008-01-11     Revised Date:  2008-05-28    
Medline Journal Info:
Nlm Unique ID:  101241539     Medline TA:  Hybridoma (Larchmt)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  302-10     Citation Subset:  IM    
Affiliation:
Immunology Section, Adolfo Lutz Institute, São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial / biosynthesis*,  therapeutic use
Antibodies, Monoclonal / biosynthesis*,  therapeutic use
Bacteremia / epidemiology,  immunology,  therapy
Brazil
Female
Humans
Immunization*
Lymph Nodes / immunology,  metabolism*
Meningococcal Infections / epidemiology,  immunology,  therapy
Mice
Mice, Inbred BALB C
Neisseria meningitidis / immunology*
Prevalence
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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