Document Detail


Production of medium-chain triacylglycerols from corn oil: optimization by response surface methodology.
MedLine Citation:
PMID:  20576567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Structured lipids (SLs) having long-chain fatty acids at sn-2 and medium-chain caprylic acid (CA, 8:0) at their sn-1,3-positions from corn oil (CO) were obtained and optimized by response surface methodology (RSM) with a three-level, three-factor face-centered cube design. Compositions of triacylglycerol species (TAGs) in SLs were also investigated by reverse-phase high performance liquid chromatography. Lipozyme TL IM from Thermomyces lanuginosa was used for the acidolysis of CO with CA in n-hexane. The effects of substrate molar ratio, enzyme amount, and reaction time on CA incorporation into CO were optimized. The optimum conditions were 13.2% (wt.) enzyme, 3.9:1 caprylic acid/corn oil molar ratio, and 3.1 h reaction time. At optimum conditions, 21.5 +/- 0.8 mol.% caprylic acid containing SLs was obtained. This product was characterized by 50% of triacylglycerol species with equivalent carbon number (ECN) C30, C32, C36, and C38, and 50% of triacylglycerol species with ECN C42, C44, and C46.
Authors:
Tarik Oztürk; Guldem Ustun; H Ayse Aksoy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioresource technology     Volume:  101     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-06-24     Completed Date:  2010-10-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  7456-61     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Istanbul Technical University, Institute of Science and Technology, Istanbul, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Biotechnology / methods*
Corn Oil / metabolism*
Fatty Acids / analysis
Hydrolysis
Lipase / metabolism
Models, Chemical
Octanoic Acids / chemistry
Regression Analysis
Reproducibility of Results
Time Factors
Triglycerides / biosynthesis*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Octanoic Acids; 0/Triglycerides; 124-07-2/caprylic acid; 8001-30-7/Corn Oil; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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