| Production of medium-chain triacylglycerols from corn oil: optimization by response surface methodology. | |
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MedLine Citation:
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PMID: 20576567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Structured lipids (SLs) having long-chain fatty acids at sn-2 and medium-chain caprylic acid (CA, 8:0) at their sn-1,3-positions from corn oil (CO) were obtained and optimized by response surface methodology (RSM) with a three-level, three-factor face-centered cube design. Compositions of triacylglycerol species (TAGs) in SLs were also investigated by reverse-phase high performance liquid chromatography. Lipozyme TL IM from Thermomyces lanuginosa was used for the acidolysis of CO with CA in n-hexane. The effects of substrate molar ratio, enzyme amount, and reaction time on CA incorporation into CO were optimized. The optimum conditions were 13.2% (wt.) enzyme, 3.9:1 caprylic acid/corn oil molar ratio, and 3.1 h reaction time. At optimum conditions, 21.5 +/- 0.8 mol.% caprylic acid containing SLs was obtained. This product was characterized by 50% of triacylglycerol species with equivalent carbon number (ECN) C30, C32, C36, and C38, and 50% of triacylglycerol species with ECN C42, C44, and C46. |
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Authors:
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Tarik Oztürk; Guldem Ustun; H Ayse Aksoy |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioresource technology Volume: 101 ISSN: 1873-2976 ISO Abbreviation: Bioresour. Technol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-06-24 Completed Date: 2010-10-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: England |
Other Details:
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Languages: eng Pagination: 7456-61 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Istanbul Technical University, Institute of Science and Technology, Istanbul, Turkey. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biotechnology
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methods* Corn Oil / metabolism* Fatty Acids / analysis Hydrolysis Lipase / metabolism Models, Chemical Octanoic Acids / chemistry Regression Analysis Reproducibility of Results Time Factors Triglycerides / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Octanoic Acids; 0/Triglycerides; 124-07-2/caprylic acid; 8001-30-7/Corn Oil; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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