Document Detail


Production of lysophosphatidic acids by lysophospholipase D in human follicular fluids of In vitro fertilization patients.
MedLine Citation:
PMID:  10377049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lysophosphatidic acids (LPAs) are known to be normal constituents of mammalian serum, and they mimic some biological effects of the serum. We previously reported that lysophospholipase D (LPLD) was involved in the accumulation of LPAs in incubated rat plasma and serum. In this study we detected, by gas-liquid chromatography, various molecular species of LPA in follicular fluids collected from women programmed for in vitro fertilization. When the follicular fluid was incubated at 37 degrees C for 48 h, persistent increases in the amounts of LPAs were observed concomitant with decreases in the amounts of the corresponding lysophosphatidylcholines (LPCs), although the concentrations of saturated LPCs increased in the first 6 h of incubation. These results suggest that human follicular fluid has LPLD activity, and this was confirmed by experiments with follicular fluids mixed with an exogenous radioactive LPC. The LPLD showed preference for unsaturated over saturated LPCs, similar to plasma LPLD, indicating that it originated from the circulation.
Authors:
A Tokumura; M Miyake; Y Nishioka; S Yamano; T Aono; K Fukuzawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of reproduction     Volume:  61     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-09-03     Completed Date:  1999-09-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  195-9     Citation Subset:  IM    
Affiliation:
Faculty of Pharmaceutical Sciences and Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Tokushima 770-8505, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Female
Fertilization in Vitro*
Follicular Fluid / enzymology*
Humans
Kinetics
Lysophosphatidylcholines / metabolism
Lysophospholipids / biosynthesis*
Male
Phosphatidylcholine-Sterol O-Acyltransferase / metabolism
Phosphoric Diester Hydrolases / metabolism*
Chemical
Reg. No./Substance:
0/Lysophosphatidylcholines; 0/Lysophospholipids; EC 2.3.1.43/Phosphatidylcholine-Sterol O-Acyltransferase; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.1.4.39/alkylglycerophosphoethanolamine phosphodiesterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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