Document Detail


Production of lymphoid tumors in hamsters by direct implantation of normal human peripheral and umbilical cord leukocytes.
MedLine Citation:
PMID:  181336     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peripheral leukocytes from Epstein-Barr virus (EBV)-seropositive or seronegative normal adults, or human umbilical cord leukocytes infected or not infected with EBV, were directly transplanted into 50 newborn hamsters treated with anti-lymphocyte serum. Lymphoid tumors were produced after a latent period of 10-25 days in 39 animals: 8 of 14 recipients of seropositive donor leukocytes, 2 of 2 recipients of seronegative donor leukocytes, 13 of 15 recipients of EBV-infected cord leukocytes, and 16 of 19 recipients of non-infected cord leukocytes. EBV-determined nuclear antigen-positive human lymphoblastoid cell lines were established in vitro from tumors produced with seropositive donor leukocytes or EBV-infected cord leukocytes but not from tumors produced with seronegative donor leukocytes or non-infected cord leukocytes. The results indicate that EBV is not a prerequisite for in vivo production of tumors but necessary for in vitro establishment of lymphoblastoid cell lines from such tumors.
Authors:
I Miyoshi; I Kubonishi; H Uchida; S Hiraki; Y Matsuda; T Tanaka; H Masuji; K Hiraki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  18     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1976-10-01     Completed Date:  1976-10-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  67-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Antilymphocyte Serum / pharmacology
Cell Line
Chromosomes
Cricetinae
Culture Techniques
Fetal Blood
Fluorescent Antibody Technique
Herpesvirus 4, Human
Humans
Infant, Newborn
Leukemia, Experimental / immunology,  pathology
Leukemia, Lymphoid / immunology*,  pathology
Leukocyte Transfusion*
Leukocytes / ultrastructure
Lymphoma / immunology*,  pathology
Neoplasm Transplantation
Neoplasms, Experimental / immunology,  pathology
Transplantation, Heterologous
Chemical
Reg. No./Substance:
0/Antilymphocyte Serum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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