Document Detail


Production of leukotriene C4 by peripheral blood leukocytes stimulated with anti-fcepsilonRI antibody, PMA, and fMLP does not correlate with irreversible airway obstruction in asthmatics.
MedLine Citation:
PMID:  17323856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Airway remodeling has recently emerged as a major problem in an increasing percentage of patients with asthma. Reasons for great diversity in the progression of irreversible bronchoconstriction among asthmatics remain unclear.
OBJECTIVE: The aim of this study was to assess whether the potential ability of leukocytes to produce cysteinyl leukotrienes in response to various stimuli is correlated with magnitude of irreversible airway obstruction in asthmatics.
MATERIALS AND METHODS: The study sample comprised 76 asthmatics (34 males, mean +/- SD age 52 +/- 13 years), and 35 healthy controls (18 males, 38.2 +/- 15 years). Each subject underwent 2 pulmonary function tests: before and after bronchodilator administration. In addition, approximate annual decline in forced expiratory volume in 1 second (FEV1) (% of predicted) was calculated. Leukotriene C4 (LTC4) production was assessed combining a cellular antigen stimulation test and enzyme-linked immunosorbent assay using Bulhmann Laboratories AG kits. Leukocytes isolated from peripheral blood were stimulated with anti-FcepsilonRI antibody, N-formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol 12-myristate 13-acetate (PMA). In separate tubes each subject's leukocytes were tested for spontaneous LTC4 production. Finally, stimulated LTC4 production was expressed in pg/mL after subtraction of values of spontaneous production.
RESULTS: In asthmatics, baseline FVC% and FEV% values ranged from 24.4% to 122.4% (mean, 75.5%) and from 23.4% to 126.6% (mean, 74.4%), respectively. There were no significant differences between asthmatics and controls in LTC4 production stimulated by anti-FcepsilonRI antibody (P = .79), fMLP (P = .33) or PMA (P = .86). We found no correlation between stimulated LTC4 production and spirometric parameters at baseline or after bronchodilator administration or annual decline in FEV1%.
CONCLUSION: Our results do not confirm the hypothesis that airway remodeling in asthma might be related to enhanced ability of leukocytes to produce cysteinyl leukotrienes in response to various stimuli.
Authors:
J Liebhart; W Medrala; U Gladysz; W Barg; E Liebhart; R Dobek; A Dor; A Kulczak; G Gogolewski; A Bigda; B Panaszek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of investigational allergology & clinical immunology     Volume:  17     ISSN:  1018-9068     ISO Abbreviation:  J Investig Allergol Clin Immunol     Publication Date:  2007  
Date Detail:
Created Date:  2007-02-27     Completed Date:  2007-04-19     Revised Date:  2013-07-31    
Medline Journal Info:
Nlm Unique ID:  9107858     Medline TA:  J Investig Allergol Clin Immunol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  1-5     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine and Allergology, Wroclaw Medical University, Wroclaw, Poland. Jerzy.Liebhart@dilnet.wroc.pl
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MeSH Terms
Descriptor/Qualifier:
Adult
Airway Obstruction / immunology,  physiopathology
Antibodies / pharmacology*
Asthma / immunology,  physiopathology*
Cells, Cultured
Humans
Leukocytes / drug effects*,  immunology
Leukotriene C4 / biosynthesis*
Male
Middle Aged
N-Formylmethionine Leucyl-Phenylalanine / pharmacology*
Receptors, IgE / immunology
Spirometry
Tetradecanoylphorbol Acetate / pharmacology*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Receptors, IgE; 16561-29-8/Tetradecanoylphorbol Acetate; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 72025-60-6/Leukotriene C4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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