Document Detail


Production of large numbers of plasmacytoid dendritic cells with functional activities from CD34+ hematopoietic progenitor cells: use of IL-3.
MedLine Citation:
PMID:  22245566     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Plasmacytoid dendritic cells (pDC), a subset of dendritic cells characterized by a rapid and massive type I interferon secretion through the Toll-like receptor pathway in response to viral infection, play important roles in the pathogenesis of several diseases such as chronic viral infections (HCV, HIV), autoimmunity (psoriasis, SLE) and cancer. As pDC represent a rare cell type in the peripheral blood, the goal of this study was to develop a new method to efficiently generate large numbers of cells from a limited number of CD34+ cord blood progenitors in order to provide a tool to resolve important questions about how pDC mediate tolerance, autoimmunity and cancer. Human CD34+ hematopoietic progenitor cells (HPC) isolated from cord blood were cultured with a combination of Flt3-Ligand (Flt3L), thrombopoietin (TPO) and one of the following cytokine: interleukin-3 (IL-3), interferon-β (IFNβ) or prostaglandin (PG)E2. Cells obtained in the different culture conditions were analyzed for their phenotype and functional characteristics. The addition of IL-3 cooperates with Flt3L and TPO in the induction of pDC from CD34+ HPC. Indeed, Flt3L/TPO alone or supplemented with PGE2 or IFNβ produced smaller amounts of pDC from HPC. Moreover, pDC generated in Flt3L/TPO/IL-3 cultures exhibited morphological, immunohistochemical and functional features of peripheral blood pDC. We showed that IL-3, in association with Flt3L and TPO, provides an advantageous tool for the large-scale generation of pDC. Indeed, this culture condition generated, starting from 2x105 CD34+ cells, up to 2.6x106 pDC presenting features of blood pDC.
Authors:
Stéphanie Demoulin; Patrick Roncarati; Philippe Delvenne; Pascale Hubert
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-10
Journal Detail:
Title:  Experimental hematology     Volume:  -     ISSN:  1873-2399     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.
Affiliation:
Laboratory of Experimental Pathology, Bât.B23 Anatomie et cytologie pathologiques Tour 3 +4, Avenue de l'Hôpital 3, 4000 Liège 1, Belgium.
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