| Production of interleukin 6 by hepatoma cells. | |
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MedLine Citation:
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PMID: 1699111 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Five rat hepatoma cell lines were shown to secrete hepatocyte-stimulating factors (HSFs) capable of inducing a characteristic spectrum of acute phase genes. Three of these lines, but not normal rat livers or livers from rats with an experimentally induced acute inflammation, produced interleukin 6 (IL6) mRNA. An anti-rat IL6 serum was prepared against synthetic peptides derived from the rat IL6 cDNA sequence. This antiserum cleared authentic rat IL6 and a fraction of the HSF activities secreted by the hepatoma cell lines. After concentration of culture supernatants from FTO2B hepatoma cells, IL6 was detected with the anti-IL6 serum by immuno-blot analysis. Biosynthesis of IL6 in the HTC line was demonstrated by metabolic labeling and immunoprecipitation. Secretion of HSF activities by hepatoma cells was increased by serum factors. These data suggest that different rat hepatoma lines each secrete different characteristic sets of HSF activities and establish unambiguously that IL6 is secreted by at least some of these lines. |
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Authors:
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W Northemann; M Hattori; G Baffet; T A Braciak; R G Fletcher; L J Abraham; J Gauldie; M Baumann; G H Fey |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular biology & medicine Volume: 7 ISSN: 0735-1313 ISO Abbreviation: Mol. Biol. Med. Publication Date: 1990 Jun |
Date Detail:
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Created Date: 1990-11-06 Completed Date: 1990-11-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8403879 Medline TA: Mol Biol Med Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 273-85 Citation Subset: IM |
Affiliation:
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Department of Immunology, Research Institute of Scripps Clinic La Jolla, CA 92037. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute-Phase Proteins
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biosynthesis Animals DNA / genetics Gene Expression Regulation, Neoplastic / drug effects Immunoelectrophoresis Interleukin-6 / genetics, pharmacology, secretion* Liver Neoplasms, Experimental / pathology*, secretion Neoplasm Proteins / secretion* Peptide Fragments / chemical synthesis, pharmacology Polymerase Chain Reaction RNA, Messenger / biosynthesis RNA, Neoplasm / biosynthesis Rats Tumor Cells, Cultured / secretion |
| Grant Support | |
ID/Acronym/Agency:
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AI22166/AI/NIAID NIH HHS; AI23351/AI/NIAID NIH HHS; AI27493/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acute-Phase Proteins; 0/Interleukin-6; 0/Neoplasm Proteins; 0/Peptide Fragments; 0/RNA, Messenger; 0/RNA, Neoplasm; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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