Document Detail

Production of interleukin-1 alpha and interleukin-2 by separate, phenotypically different leukaemia and human T cell lymphotropic virus-1-transformed T cell clones.
MedLine Citation:
PMID:  8314280     Owner:  NLM     Status:  MEDLINE    
The relationship between interleukin-1 (IL-1) and interleukin-2 (IL-2) production and immunophenotype marker profiles was studied in a panel of 29 leukaemia and human T cell lymphotropic virus-1 (HTLV-1)-transformed T cell lines. Culture supernatants from six of the 29 T cell lines tested increased IL-2 production by the MOLT-16 cell line in a manner similar to that of rIL-1 alpha or rIL-1 beta. The enhancing activity in the cell culture supernatants was inhibited by antibody against IL-1 alpha. Anti-IL-1 beta antibody had no inhibitory effect. All the cell lines producing IL-1 alpha had characteristics of activated mature T cells. They were terminal deoxyribonucleotidyl transferase (TdT)-, CD4+, CD8-, HLA-DR+ and all were strongly positive for IL-2R alpha (Tac antigen) expression. However, none of the IL-1 alpha producing cell lines secreted detectable IL-2. A significant quantity of IL-2 was found, after stimulation with phytohaemagglutinin, in supernatants from nine of the 29 cell lines tested. The majority of IL-2 producing cell lines originated from less mature, non-activated T cells, as they were characterized by the expression of TdT, lack of HLA-DR antigens and > 50% had no detectable IL-2R alpha. The results thus show that separate, phenotypically different leukaemia and HTLV-1-transformed T cell clones produce IL-1 alpha and IL-2.(ABSTRACT TRUNCATED AT 250 WORDS)
V Holán; J Minowada
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology and cell biology     Volume:  71 ( Pt 6)     ISSN:  0818-9641     ISO Abbreviation:  Immunol. Cell Biol.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-03-24     Completed Date:  1994-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8706300     Medline TA:  Immunol Cell Biol     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  509-15     Citation Subset:  IM; X    
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague.
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MeSH Terms
Antibodies / immunology
Cell Line, Transformed
Culture Media, Conditioned
DNA Nucleotidylexotransferase / immunology
Fluorescent Antibody Technique
Human T-lymphotropic virus 1 / physiology
Interferon-alpha / immunology
Interleukin-1 / analysis,  biosynthesis*
Interleukin-2 / analysis,  biosynthesis*
Lymphocyte Activation
Neutralization Tests
T-Lymphocytes / immunology,  metabolism*
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / immunology
Reg. No./Substance:
0/Antibodies; 0/Culture Media, Conditioned; 0/Interferon-alpha; 0/Interleukin-1; 0/Interleukin-2; 0/Tumor Necrosis Factor-alpha; EC Nucleotidylexotransferase

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