| Production of immunogenic HIV-1 viruslike particles in stably engineered monkey cell lines. | |
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MedLine Citation:
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PMID: 1707639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A proviral fragment from human immunodeficiency virus type 1 (HIV-1) (LAV-1BRU) containing only protein-coding information, was expressed in COS cells using constitutive promoters in transient and stable transfection experiments. The presence of viruslike particles in cell supernatants was verified by Western blot analysis, density gradient centrifugation, and electron microscopy. Transfection of Vero cells with a similar construct employing the human metallothionein promoter led to the isolation of stable cell lines exhibiting inducible viruslike particle expression in response to cadmium chloride treatment. Induction ratios for viruslike particle expression were in excess of 1000-fold with production levels of p24 core antigen as high as 0.6 mg/L per 24 h. HIV-1 viruslike particles were immunogenic in mice, leading to strong envelope and core-specific humoral responses after two immunizations. The development of stable cell lines expressing significant quantities of HIV-1 viruslike particles offers an alternative to the use of live virus vectors for the production and evaluation of particle-based AIDS vaccines. |
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Authors:
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J R Haynes; S X Cao; B Rovinski; C Sia; O James; G A Dekaban; M H Klein |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: AIDS research and human retroviruses Volume: 7 ISSN: 0889-2229 ISO Abbreviation: AIDS Res. Hum. Retroviruses Publication Date: 1991 Jan |
Date Detail:
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Created Date: 1991-05-21 Completed Date: 1991-05-21 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8709376 Medline TA: AIDS Res Hum Retroviruses Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 17-27 Citation Subset: IM; X |
Affiliation:
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Connaught Centre for Biotechnology Research, Willowdale, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cell Line Centrifugation, Density Gradient Female Gene Products, gag / biosynthesis HIV Antibodies / biosynthesis, immunology HIV Antigens / biosynthesis* HIV Core Protein p24 HIV-1 / growth & development*, immunology, ultrastructure Haplorhini Humans Metallothionein / genetics Mice Mice, Inbred C3H Mice, Inbred C57BL Plasmids Promoter Regions, Genetic RNA-Directed DNA Polymerase / metabolism Transfection* Vero Cells Viral Core Proteins / biosynthesis Virion / growth & development*, immunology, pathogenicity, ultrastructure Virus Cultivation |
| Chemical | |
Reg. No./Substance:
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0/Gene Products, gag; 0/HIV Antibodies; 0/HIV Antigens; 0/HIV Core Protein p24; 0/Viral Core Proteins; 9038-94-2/Metallothionein; EC 2.7.7.49/RNA-Directed DNA Polymerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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