Document Detail

Production of hydroxyl radical in the hippocampus after CO hypoxia or hypoxic hypoxia in the rat.
MedLine Citation:
PMID:  9013136     Owner:  NLM     Status:  MEDLINE    
Carbon monoxide poisoning produces both immediate and delayed neuronal injury in selective regions of the brain that is not readily explained on the basis of tissue hypoxia. One possibility is that cellular injury during and after CO poisoning is related to the production of reactive oxygen species (ROS) by the brain. In this study, we hypothesized that the extent of ROS generation in the brain would be greater after CO than after hypoxic hypoxia due to intracellular uptake of CO. We assessed hydroxyl radical (OH.) production by comparing the nonenzymatic hydroxylation of salicylic acid to 2,3-dihydroxybenzoic acid (2,3-DHBA) in the hippocampus of the rat by microdialysis during either CO hypoxia or an exposure to hypoxic hypoxia that produced similar PO2 and cerebral blood flow (CBF) values in the region of microdialysis. We found neither control animals nor animals exposed to 30 min of hypoxic hypoxia at a mean tissue PO2 of 15 mmHg demonstrated significant increases in 2,3-DHBA production in the hippocampus over the 2-h the exposure. In contrast, CO exposed rats which also developed brain PO2 values in the range of 15 mmHg showed highly significant increases in 2,3-DHBA production. We conclude that cerebral oxidative stress in the hippocampus of the rat during CO hypoxia in vivo is not a direct effect of decreased tissue oxygen concentration.
C A Piantadosi; J Zhang; I T Demchenko
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  22     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  1997  
Date Detail:
Created Date:  1997-04-17     Completed Date:  1997-04-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  725-32     Citation Subset:  IM; S    
F.G. Hall Center for Hypobaric and Hyperbaric Medicine, Duke University Medical Center, Durham, NC 27710, USA.
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MeSH Terms
Anoxia / metabolism*,  physiopathology
Carbon Monoxide Poisoning / metabolism*,  physiopathology
Cerebrovascular Circulation
Hemoglobins / metabolism
Hippocampus / metabolism*
Hydroxybenzoic Acids / metabolism
Hydroxyl Radical / metabolism*
Oxidative Stress
Oxygen Consumption
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism
Salicylic Acid
Salicylic Acids / metabolism
Reg. No./Substance:
0/Hemoglobins; 0/Hydroxybenzoic Acids; 0/Reactive Oxygen Species; 0/Salicylic Acids; 303-38-8/2-pyrocatechuic acid; 3352-57-6/Hydroxyl Radical; 69-72-7/Salicylic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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