| Production of a human monoclonal antibody to normal basal and squamous cell carcinoma-associated antigen. | |
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MedLine Citation:
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PMID: 7510337 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A human monoclonal antibody, BM2, was produced by a hybridoma line generated by fusion of lymph node cells from a patient with squamous cell carcinoma (SCC) of the tongue with human B-lymphoblastoid cell line HO-323. BM2, an IgM class antibody, was reactive with all of the SCC cell lines tested. Frozen sections of normal and malignant tumor specimens were investigated to examine the reactivity of BM2 towards them. All 35 oral SCC specimens reacted with BM2. Normal stratified squamous epithelium showed positive staining in basal cells, but no staining was seen in other layers of the stratified epithelium, simple epithelium, and tissues of nonepithelial origin. Ductal basal cells of normal salivary gland also showed positive staining. Western blotting and immunoprecipitation analysis revealed that BM2 recognized 52 kDa membrane-associated protein. BM2 may therefore be a useful tool for biological and clinical studies of SCC. |
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Authors:
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M Yoshiura; H Murakami; H Tashiro; K Kurisu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology Volume: 22 ISSN: 0904-2512 ISO Abbreviation: J. Oral Pathol. Med. Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1994-04-11 Completed Date: 1994-04-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8911934 Medline TA: J Oral Pathol Med Country: DENMARK |
Other Details:
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Languages: eng Pagination: 451-8 Citation Subset: D; IM |
Affiliation:
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First Department of Oral and Maxillofacial Surgery, Kyushu University, Fukuoka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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immunology Antibodies, Monoclonal / biosynthesis*, drug effects Antigens, Neoplasm / immunology* Carcinoma, Squamous Cell / immunology* Epithelial Cells Epithelium / immunology Fluorescent Antibody Technique Formaldehyde / pharmacology Humans Hybridomas / immunology Immunoglobulin Isotypes / biosynthesis, drug effects Immunoglobulin M / biosynthesis, drug effects Immunohistochemistry Keratins Membrane Proteins / immunology Mitosis / immunology Mouth Mucosa / cytology, immunology* Neuraminidase / pharmacology Periodic Acid / pharmacology Pronase / pharmacology Submandibular Gland / cytology, immunology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/Immunoglobulin Isotypes; 0/Immunoglobulin M; 0/Membrane Proteins; 10450-60-9/Periodic Acid; 50-00-0/Formaldehyde; 68238-35-7/Keratins; 7790-28-5/metaperiodate; EC 3.2.1.18/Neuraminidase; EC 3.4.24.-/Pronase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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