Document Detail


Production of a human monoclonal antibody to normal basal and squamous cell carcinoma-associated antigen.
MedLine Citation:
PMID:  7510337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human monoclonal antibody, BM2, was produced by a hybridoma line generated by fusion of lymph node cells from a patient with squamous cell carcinoma (SCC) of the tongue with human B-lymphoblastoid cell line HO-323. BM2, an IgM class antibody, was reactive with all of the SCC cell lines tested. Frozen sections of normal and malignant tumor specimens were investigated to examine the reactivity of BM2 towards them. All 35 oral SCC specimens reacted with BM2. Normal stratified squamous epithelium showed positive staining in basal cells, but no staining was seen in other layers of the stratified epithelium, simple epithelium, and tissues of nonepithelial origin. Ductal basal cells of normal salivary gland also showed positive staining. Western blotting and immunoprecipitation analysis revealed that BM2 recognized 52 kDa membrane-associated protein. BM2 may therefore be a useful tool for biological and clinical studies of SCC.
Authors:
M Yoshiura; H Murakami; H Tashiro; K Kurisu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  22     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-04-11     Completed Date:  1994-04-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  451-8     Citation Subset:  D; IM    
Affiliation:
First Department of Oral and Maxillofacial Surgery, Kyushu University, Fukuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / immunology
Antibodies, Monoclonal / biosynthesis*,  drug effects
Antigens, Neoplasm / immunology*
Carcinoma, Squamous Cell / immunology*
Epithelial Cells
Epithelium / immunology
Fluorescent Antibody Technique
Formaldehyde / pharmacology
Humans
Hybridomas / immunology
Immunoglobulin Isotypes / biosynthesis,  drug effects
Immunoglobulin M / biosynthesis,  drug effects
Immunohistochemistry
Keratins
Membrane Proteins / immunology
Mitosis / immunology
Mouth Mucosa / cytology,  immunology*
Neuraminidase / pharmacology
Periodic Acid / pharmacology
Pronase / pharmacology
Submandibular Gland / cytology,  immunology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/Immunoglobulin Isotypes; 0/Immunoglobulin M; 0/Membrane Proteins; 10450-60-9/Periodic Acid; 50-00-0/Formaldehyde; 68238-35-7/Keratins; 7790-28-5/metaperiodate; EC 3.2.1.18/Neuraminidase; EC 3.4.24.-/Pronase

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