Document Detail


Production of glucolipids and specialty fatty acids from sophorolipids by Penicillium decumbens naringinase: Optimization and kinetics.
MedLine Citation:
PMID:  19194976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In view of global environmental concerns and the awakening to the exhaustibility of our natural resources, an increasing importance of biologically derived surfactants can be expected in the near future. Enzymatic modification of these biosurfactants allows to improve their characteristics and so extend their application field. In view of this, glucolipids are interesting substrates e.g., for the synthesis of new glycolipids with increased biological activity. Here, we describe the optimization of glucolipid production from Candida bombicola sophorolipids by Penicillium decumbens naringinase and show that the enzyme might be useful for production of specialty fatty acids as well. Optimum conditions for production of glucolipids were found to be pH 7.0 and 50 degrees C with a yield of 80% (w/w) glucolipids after 3 h of incubation. The K(m) for sophorolipids was 1.67 mM, while V(max) was 0.035 mM sophorolipids/min. At pH 3.0, glucolipids were immediately further hydrolyzed and completely converted to fatty acids after 24 h of incubation, offering a biological route to the synthesis of unique specialty fatty acids. The K(m) for glucolipids was 11 mM while V(max) was 0.21 mM glucolipids/min. Glucose inhibited the enzyme in a competitive way with K(I) around 10-15 mM glucose. Surfactant properties of the produced glucolipids were comparable to those of the acidic sophorolipids.
Authors:
Karen Saerens; Inge Van Bogaert; Wim Soetaert; Erick Vandamme
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology journal     Volume:  4     ISSN:  1860-7314     ISO Abbreviation:  Biotechnol J     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-21     Completed Date:  2009-06-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  517-24     Citation Subset:  IM    
Affiliation:
Ghent University, Belgium. Karen.Saerens@UGent.be
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MeSH Terms
Descriptor/Qualifier:
Candida / chemistry
Fatty Acids / biosynthesis*
Glycolipids / biosynthesis*,  chemistry,  metabolism
Hydrogen-Ion Concentration
Kinetics
Multienzyme Complexes / metabolism*
Penicillium / enzymology*
Surface-Active Agents / chemistry,  metabolism*
Temperature
Time Factors
beta-Glucosidase / metabolism*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Glycolipids; 0/Multienzyme Complexes; 0/Surface-Active Agents; EC 3.2.1.-/naringinase; EC 3.2.1.21/beta-Glucosidase
Comments/Corrections
Comment In:
Biotechnol J. 2009 Apr;4(4):439   [PMID:  19370730 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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