| Production of glucaric acid from a synthetic pathway in recombinant Escherichia coli. | |
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MedLine Citation:
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PMID: 19060162 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A synthetic pathway has been constructed for the production of glucuronic and glucaric acids from glucose in Escherichia coli. Coexpression of the genes encoding myo-inositol-1-phosphate synthase (Ino1) from Saccharomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice led to production of glucuronic acid through the intermediate myo-inositol. Glucuronic acid concentrations up to 0.3 g/liter were measured in the culture broth. The activity of MIOX was rate limiting, resulting in the accumulation of both myo-inositol and glucuronic acid as final products, in approximately equal concentrations. Inclusion of a third enzyme, uronate dehydrogenase (Udh) from Pseudomonas syringae, facilitated the conversion of glucuronic acid to glucaric acid. The activity of this recombinant enzyme was more than 2 orders of magnitude higher than that of Ino1 and MIOX and increased overall flux through the pathway such that glucaric acid concentrations in excess of 1 g/liter were observed. This represents a novel microbial system for the biological production of glucaric acid, a "top value-added chemical" from biomass. |
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Authors:
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Tae Seok Moon; Sang-Hwal Yoon; Amanda M Lanza; Joseph D Roy-Mayhew; Kristala L Jones Prather |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-05 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 75 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-26 Completed Date: 2009-02-16 Revised Date: 2012-05-25 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 589-95 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde Oxidoreductases
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genetics,
metabolism Animals Escherichia coli / genetics, metabolism* Glucaric Acid / metabolism* Inositol / metabolism Inositol Oxygenase / genetics, metabolism Metabolic Networks and Pathways / genetics* Mice Myo-Inositol-1-Phosphate Synthase / genetics, metabolism Pseudomonas syringae / genetics Recombinant Proteins / genetics, metabolism Saccharomyces cerevisiae / genetics |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 25525-21-7/Glucaric Acid; 6917-35-7/Inositol; EC 1.1.1.203/uronate dehydrogenase; EC 1.13.99.1/Inositol Oxygenase; EC 1.2.-/Aldehyde Oxidoreductases; EC 5.5.1.4/Myo-Inositol-1-Phosphate Synthase |
| Comments/Corrections | |
Erratum In:
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Appl Environ Microbiol. 2009 Jul;75(13):4660 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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