Document Detail


Production of giant cells of Escherichia coli.
MedLine Citation:
PMID:  342516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Giant cells, with volumes up to 500-fold those of normal cells, have been produced by both genetic and pharmacological means in Escherichia coli K-12. In the genetic approach, an envB or mon mutation (conferring rounded or irregular morphology) was combined with a lon mutation (block of septation after irradiation). UV irradiation and subsequent incubation for 2 to 5 h in a rich medium supplemented with 1% sodium chloride led t; production of polymorphic giant cells. In the pharmacological approach, incubation of several different strains of E. coli K-12 with the drug 6-amidinopenicillanic acid (FL1060) in the same rich medium gave rise to a homogeneous population of smoothly rounded giant cells.
Authors:
W S Long; C L Slayman; K B Low
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  133     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1978 Feb 
Date Detail:
Created Date:  1978-04-26     Completed Date:  1978-04-26     Revised Date:  2010-09-02    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  995-1007     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Escherichia coli / cytology*,  genetics,  radiation effects
Microscopy, Phase-Contrast
Mutation
Phenotype
Transduction, Genetic
Ultraviolet Rays
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