| Production of gamma-linolenic acid by disrupted mycelia of Mortierella isabellina. | |
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MedLine Citation:
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PMID: 12581380 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To optimize the production of linolenic acid by disrupted mycelia of Mortierella isabellina. METHODS AND RESULTS: Effects of incubation conditions such as incubation time, pH of reaction mixture, concentration of Mg2+ or malate and incubation temperature on production of linolenic acid were studied. The production of gamma-linolenic acid reached 224 mg g-1 dry cells when the reaction mixture was composed of 1.0 g (dry mycelial mass) of disrupted mycelia of M. isabellina, 50 ml (50 mmol l(-1)) potassium phosphate buffer supplemented with 0.312 mmol l(-1) of Mg2+ and 10 mmol l(-1) of malate, pH 7.0 and incubated at 5 degrees C for 1 day. CONCLUSIONS: Incubation temperature, concentration of Mg2+ and malate showed major effects on the increased linolenic acid production. SIGNIFICANCE AND IMPACT OF THE STUDY: This study highlights conditions for increasing gamma-linolenic acid production by cell-free mycelia of M. isabellina and an insight into rapidly gaining high production of polyunsaturated fatty acids. |
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Authors:
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M Xian; J Nie; Q Meng; J Liu; C Zhou; Y Kang; K Zhen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Letters in applied microbiology Volume: 36 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2003 |
Date Detail:
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Created Date: 2003-02-12 Completed Date: 2003-05-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 182-5 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Jilin University, Changchun 130023, China. xianmo@263.net |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Culture Media Fatty Acid Desaturases / metabolism Magnesium / metabolism Malates / metabolism Mortierella / enzymology, growth & development, metabolism* Mycelium / cytology, enzymology, metabolism Temperature gamma-Linolenic Acid / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Malates; 506-26-3/gamma-Linolenic Acid; 6915-15-7/malic acid; 7439-95-4/Magnesium; EC 1.14.19.-/Fatty Acid Desaturases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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