Document Detail

Production of fermented milk using a malty compound-producing strain of Lactococcus lactis subsp. lactis biovar. diacetylactis, isolated from Zimbabwean naturally fermented milk.
MedLine Citation:
PMID:  9631339     Owner:  NLM     Status:  MEDLINE    
Malty compound-producing Lactococcus lactis subsp. lactis biovar. diacetylactis strain INF-DM1, originally isolated from naturally fermented milk in Zimbabwe was used to prepare fermented milk from ordinary milk, milk enriched with 2.5% (w/v) skimmed milk powder and by 2.5% (w/v) increase in dry matter by ultrafiltration. Inoculated milk was incubated at 22, 30 and 37 degrees C. Analyses were made after 0, 9, 18 and 24 h incubation and also after 24 h incubation followed by storage for one week at 4 degrees C. Samples were analysed for volatile compounds including malty compounds and for organic acids, pH and log cfu/g. All samples were also judged for sensory attributes. Products made from enriched milks showed increased viscosity which was most marked in ultrafiltrated milk incubated at 30 and 37 degrees C. The levels of certain compounds (lactic acid, citrate and diacetyl) were significantly affected by milk type. Incubation temperature had a significant effect on starter growth rate and the rate of production and amount of the malty compounds, lactate, diacetyl, ethanol, acetoin and acetaldehyde. 3-Methyl butanal concentrations were above the taste threshold level of 0.06 ppm in almost all products, including stored products. Although initial growth rate was fastest at 37 degrees C, an uncoupling of acid production and growth was observed after 9 h incubation, suggesting that this is above the optimum temperature for this strain. In addition, products incubated at 37 degrees C showed a tendency to whey separation, indicating that this temperature is also too high to give optimum product quality. All products attained good scores in sensory analysis provided that fermentation was complete. Variation in the levels of malty compounds during the fermentation had no significant effect on the sensory score for total flavour.
J A Narvhus; K Osteraas; T Mutukumira; R K Abrahamsen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of food microbiology     Volume:  41     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-07-21     Completed Date:  1998-07-21     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  73-80     Citation Subset:  IM    
Department of Food Science, Agricultural University of Norway, Aas, Norway.
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MeSH Terms
Acetaldehyde / analysis
Acetoin / analysis
Aldehydes / analysis
Chromatography, Gas
Citric Acid / analysis
Colony Count, Microbial
Diacetyl / analysis
Ethanol / analysis
Lactic Acid / analysis
Lactococcus lactis / metabolism*
Milk / microbiology*
Reg. No./Substance:
0/Aldehydes; 431-03-8/Diacetyl; 50-21-5/Lactic Acid; 513-86-0/Acetoin; 590-86-3/isovalerylaldehyde; 64-17-5/Ethanol; 75-07-0/Acetaldehyde; 77-92-9/Citric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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