Document Detail


Production of an extracellular thermohalophilic lipase from a moderately halophilic bacterium, Salinivibrio sp. strain SA-2.
MedLine Citation:
PMID:  18506896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fifty strains of moderately halophilic bacteria were isolated from various salty environments in Iran. A strain designated as SA-2 was shown to be the best producer of extracellular lipase and was selected for further studies. Biochemical and physiological characterization along with 16S rDNA sequence analysis placed SA-2 in the genus Salinivibrio. The optimum salt, pH, temperature and aeration for enzyme production were 0.1 M KCl, pH 8, 35 degrees C and 150 rpm, respectively. The enzyme production was synchronized bacterial growth and reached a maximum level during the early-stationary phase in the basal medium containing 1 M NaCl. Triacylglycerols enhanced lipase production, while carbohydrates had inhibitory effects on it. The maximum lipase activity was obtained at pH 7.5, 50 degrees C and CaCl(2) concentration of 0.01 M. The enzyme was stable at pH range of 7.5-8 and retained 90% of its activity at 80 degrees C for 30 min. Different concentrations of NaNO(3), Na(2)SO(4), KCl and NaCl had no affect on lipase stability for 3 h. These results suggest that the lipase secreted by Salinivibrio sp. strain SA-2 is industrially important from the perspective of its tolerance to a broad temperature range, its moderate thermoactivity and its high tolerance to a wide range of salt concentrations (0-3 M NaCl).
Authors:
Mohammad Ali Amoozegar; Ensieh Salehghamari; Khosro Khajeh; Mahbube Kabiri; Saied Naddaf
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of basic microbiology     Volume:  48     ISSN:  0233-111X     ISO Abbreviation:  J. Basic Microbiol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-03     Completed Date:  2008-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8503885     Medline TA:  J Basic Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  160-7     Citation Subset:  IM    
Affiliation:
Extremophiles Laboratory, Department of Microbiology, Faculty of Biology, College of Science, University of Tehran, Tehran, Iran. amozegar@khayam.ut.ac.ir
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / biosynthesis*,  metabolism*
Culture Media
DNA, Bacterial / isolation & purification
DNA, Ribosomal / isolation & purification
Enzyme Stability
Hydrogen-Ion Concentration
Iran
Kinetics
Lipase / biosynthesis*,  metabolism
Phylogeny
Polymerase Chain Reaction
Potassium Chloride / metabolism
RNA, Ribosomal, 16S / isolation & purification
Sequence Analysis, DNA
Sodium Chloride / metabolism
Soil Microbiology
Temperature
Vibrionaceae / enzymology*,  growth & development,  isolation & purification
Water Microbiology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S; 7447-40-7/Potassium Chloride; 7647-14-5/Sodium Chloride; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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