| Production of extracellular polysaccharide by Bacillus megaterium RB-05 using jute as substrate. | |
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MedLine Citation:
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PMID: 21507629 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Bacillus megaterium RB-05 was grown on glucose and on "tossa-daisee" (Corchorus olitorius)-derived jute, and production and composition of extracellular polysaccharide (EPS) were monitored. An EPS yield of 0.065±0.013 and of 0.297g±0.054g(-1) substrate after 72h was obtained for glucose and jute, respectively. EPS production in the presence of jute paralleled bacterial cellulase activity. High performance liquid chromatography (HPLC), matrix assisted LASER desorption/ionization-time of flight (MALDI-ToF) mass spectroscopy, and fourier transform infrared (FT-IR) spectroscopy demonstrated that the EPS synthesized in jute culture (JC) differed from that synthesized in glucose mineral salts medium (GMSM). While fucose was only a minor constituent (4.9wt.%) of EPS from GMSM, it a major component (41.9wt.%) of EPS synthesized in JC. This study establishes jute as an effective fermentation substrate for EPS production by a cellulase-producing bacterium. |
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Authors:
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Sougata Roy Chowdhury; Ratan Kumar Basak; Ramkrishna Sen; Basudam Adhikari |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-5 |
Journal Detail:
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Title: Bioresource technology Volume: - ISSN: 1873-2976 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
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Materials Science Centre, Indian Institute of Technology Kharagpur, West Bengal 721302, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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