Document Detail

Production of an extracellular polysaccharide bioflocculant by Klebsiella pneumoniae.
MedLine Citation:
PMID:  10664839     Owner:  NLM     Status:  MEDLINE    
Klebsiella pneumoniae H12 produced a newly identified extracellular polysaccharide in an ethanol medium with a yield of 3.0 g/l. The molar composition of the polysaccharide was 56.04% galactose, 25.92% glucose, 10.92% galacturonic acid, 3.71% mannose, and 3.37% glucuronic acid. The addition of 0.5%-1.5% NaCl increased production. The polysaccharide flocculated with kaolin clay in suspension at the concentration of 1 ppm in a 300-ppm solution of CaCl2. Almost all bacterial species cells aggregated in the polysaccharide solution. The ability to flocculate with kaolin clay changed with the pH and with the concentrations of coexisting cation and anion species. The polysaccharide flocculant may participate in in vivo bacterial aggregation or adherence to host organisms.
K Nakata; R Kurane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  63     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-03-21     Completed Date:  2000-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  2064-8     Citation Subset:  IM    
Central Research Laboratories, Mercian Corporation, Fujisawa, Japan.
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MeSH Terms
Flocculation Tests
Klebsiella pneumoniae / classification,  metabolism*
Polysaccharides, Bacterial / biosynthesis*,  chemistry
Reg. No./Substance:
0/Polysaccharides, Bacterial; 64-17-5/Ethanol

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