| Production of a dimer of 2-acetylaminofluorene during the sulfation of N-hydroxy-2-acetylaminofluorene in vitro. | |
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MedLine Citation:
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PMID: 40769 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During the sulfation of N-hydroxy-2-acetylaminofluorene (NOH-2AAF) by rat liver 100,000 g supernatant fraction in vitro, an unidentified metabolite is produced which accounts for 22% of the N-OH-2AAF metabolized. This product has been characterized as the 2AAF dimer, 1-(N-2'-fluorenylacetamido-2-acetylaminofluorene) by comparing its TLC, HPLC, UV, and mass spectral properties with a synthetic standard which was prepared from the reaction of N-acetoxy-2-acetylaminofluorene (N-AcO-2AAF) with 2AAF. Increasing amounts of 2AAF added to the incubation mixture of N-OH-[acetyl-14C]2AAF and rat liver 100,000 g supernatant fraction decreased the irreversible binding of 14C to protein, and increased the formation of 2AAF dimer proportionately. This suggests that the 2AAF dimer is formed from the reaction of 2AAF and the electrophilic species produced from the sulfated N-OH-2AAF. In the presence of the 9,000 g fraction of rat liver, the dimer of 2AAF was aroximately 1/25 as active as 2AAF in producing mutations in the Salmonella mutagenesis test system. |
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Authors:
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L S Andrews; L R Pohl; J A Hinson; C L Fisk; J R Gillette |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Drug metabolism and disposition: the biological fate of chemicals Volume: 7 ISSN: 0090-9556 ISO Abbreviation: Drug Metab. Dispos. Publication Date: 1979 Sep-Oct |
Date Detail:
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Created Date: 1980-01-28 Completed Date: 1980-01-28 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9421550 Medline TA: Drug Metab Dispos Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 296-300 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2-Acetylaminofluorene
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metabolism*,
pharmacology Animals Biotransformation Male Mutagens Polymers Rats Salmonella / drug effects, genetics Sulfates / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Mutagens; 0/Polymers; 0/Sulfates; 53-96-3/2-Acetylaminofluorene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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