| Production of a diagnostic monoclonal antibody in perennial alfalfa plants. | |
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MedLine Citation:
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PMID: 10397849 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The increasing use of monoclonal antibodies (mAbs) in diagnostic reagents necessitates efficient and cost-effective mAb production methods. In blood banks, one of the most routinely used reagents is the anti-human IgG reagent used for the detection of non-agglutinating antibodies. Here we report the production of a functional, purified anti-human IgG, through the expression of its encoding genes in perennial transgenic alfalfa. Transgenic plants expressing the light- and heavy-chain encoding mRNAs were obtained, and plants from crosses were found to express fully assembled C5-1. The purification procedure yielded mainly the H2L2 form with specificity and affinity identical to those of hybridoma-derived C5-1. The ability to accumulate the antibody was maintained both in parental F1 lines during repeated harvesting and in clonal material; the antibody was stable in the drying hay as in extracts made in pure water. Also, plant and hybridoma-derived C5-1 had similar in vivo half-lives in mice. These results indicate that plant C5-1 could be used in a diagnostic reagent as effectively as hybridoma-derived C5-1, and demonstrates the usefulness of perennial systems for the cost-effective, stable, and reliable production of large amounts of mAbs. |
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Authors:
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H Khoudi; S Laberge; J M Ferullo; R Bazin; A Darveau; Y Castonguay; G Allard; R Lemieux; L P Vézina |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 64 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1999 Jul |
Date Detail:
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Created Date: 1999-09-13 Completed Date: 1999-09-13 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 135-43 Citation Subset: IM |
Copyright Information:
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Copyright 1999 John Wiley & Sons, Inc. |
Affiliation:
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Plant Science Department, FSAA, Université Laval, Sainte-Foy, Québec G1K 7P4. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / biosynthesis* Chromatography, Affinity Genes, Plant Humans Hybridomas Medicago sativa / genetics*, immunology Mice Plants, Genetically Modified Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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